A simple filtration technique for obtaining purified human chromosomes in suspension

Here we present a simple method for cleaning polyamine human mitotic chromosomes in solution. This was achieved by filtering intact (unburst) nuclei along with both large and small cytoplasmic debris through a series of different pore sized filters. Pure human chromosomes were recovered using a simp...

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Bibliographic Details
Main Authors: Mohammed Yusuf, Neha Parmar, Gurdeep K. Bhella, Ian K Robinson
Format: Article
Language:English
Published: Taylor & Francis Group 2014-05-01
Series:BioTechniques
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Online Access:https://www.future-science.com/doi/10.2144/000114168
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