Melatonin Supplementation Enhances Next-Day High-Intensity Exercise Performance and Recovery in Trained Males: A Placebo-Controlled Crossover Study

Background/Objectives: Sleep and recovery are critical for optimising exercise performance. However, the efficacy of melatonin supplementation in improving sleep quality and next-day physical performance remains unclear. This study examined the effects of melatonin ingestion on sleep and performance...

Full description

Saved in:
Bibliographic Details
Main Authors: Nourhène Mahdi, Slaheddine Delleli, Arwa Jebabli, Khouloud Ben Maaoui, Juan Del Coso, Hamdi Chtourou, Luca Paolo Ardigò, Ibrahim Ouergui
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Sports
Subjects:
Online Access:https://www.mdpi.com/2075-4663/13/6/190
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849431728531177472
author Nourhène Mahdi
Slaheddine Delleli
Arwa Jebabli
Khouloud Ben Maaoui
Juan Del Coso
Hamdi Chtourou
Luca Paolo Ardigò
Ibrahim Ouergui
author_facet Nourhène Mahdi
Slaheddine Delleli
Arwa Jebabli
Khouloud Ben Maaoui
Juan Del Coso
Hamdi Chtourou
Luca Paolo Ardigò
Ibrahim Ouergui
author_sort Nourhène Mahdi
collection DOAJ
description Background/Objectives: Sleep and recovery are critical for optimising exercise performance. However, the efficacy of melatonin supplementation in improving sleep quality and next-day physical performance remains unclear. This study examined the effects of melatonin ingestion on sleep and performance-related outcomes the following day in trained males. Methods: In a randomised, double-blind, placebo-controlled crossover study, 12 trained males (age: 21.92 ± 2.84 years) ingested 6 mg of melatonin (MEL) or a placebo (PLA) the night before performing the 5 m shuttle test (5mSRT). Before and after the 5mSRT, blood samples were collected. Peak heart rate (HR<sub>peak</sub>) and rating of perceived exertion (RPE) were recorded throughout the test. Perceived recovery status (PRS) and delayed onset muscle soreness (DOMS) were measured before, 5 min, 24 h, 48 h, and 72 h after the test. The sleep/wake cycle was monitored during the night after ingestion. Results: Data were analysed using paired t-tests, Wilcoxon tests, and two-way ANOVAs, with significance set at <i>p</i> < 0.05. Compared to PLA, MEL did not modify any sleep parameters or blood markers (all <i>p</i> > 0.05). However, MEL improved total distance, fatigue index, the percentage decrement between sprints, and HR<sub>peak</sub> (all <i>p</i> < 0.05) in the 5mSRT compared to PLA. MEL also enhanced PRS values up to 72 h post-exercise and reduced DOMS (all <i>p</i> < 0.05). Conclusion: In summary, 6 mg of melatonin taken at night enhanced next-day high-intensity exercise performance and improved perceived recovery up to 72 h post-exercise.
format Article
id doaj-art-d4669201c1354b74991db7fb07d363f2
institution Kabale University
issn 2075-4663
language English
publishDate 2025-06-01
publisher MDPI AG
record_format Article
series Sports
spelling doaj-art-d4669201c1354b74991db7fb07d363f22025-08-20T03:27:33ZengMDPI AGSports2075-46632025-06-0113619010.3390/sports13060190Melatonin Supplementation Enhances Next-Day High-Intensity Exercise Performance and Recovery in Trained Males: A Placebo-Controlled Crossover StudyNourhène Mahdi0Slaheddine Delleli1Arwa Jebabli2Khouloud Ben Maaoui3Juan Del Coso4Hamdi Chtourou5Luca Paolo Ardigò6Ibrahim Ouergui7High Institute of Sport and Physical Education of Sfax, University of Sfax, Sfax 3000, TunisiaHigh Institute of Sport and Physical Education of Sfax, University of Sfax, Sfax 3000, TunisiaHigh Institute of Sport and Physical Education of Sfax, University of Sfax, Sfax 3000, TunisiaHigh Institute of Sport and Physical Education of Sfax, University of Sfax, Sfax 3000, TunisiaCentre for Sport Studies, Rey Juan Carlos University, 28943 Fuenlabrada, SpainHigh Institute of Sport and Physical Education of Sfax, University of Sfax, Sfax 3000, TunisiaDepartment of Teacher Education, NLA University College, 0166 Oslo, NorwayHigh Institute of Sport and Physical Education of Kef, University of Jendouba, Kef 7100, TunisiaBackground/Objectives: Sleep and recovery are critical for optimising exercise performance. However, the efficacy of melatonin supplementation in improving sleep quality and next-day physical performance remains unclear. This study examined the effects of melatonin ingestion on sleep and performance-related outcomes the following day in trained males. Methods: In a randomised, double-blind, placebo-controlled crossover study, 12 trained males (age: 21.92 ± 2.84 years) ingested 6 mg of melatonin (MEL) or a placebo (PLA) the night before performing the 5 m shuttle test (5mSRT). Before and after the 5mSRT, blood samples were collected. Peak heart rate (HR<sub>peak</sub>) and rating of perceived exertion (RPE) were recorded throughout the test. Perceived recovery status (PRS) and delayed onset muscle soreness (DOMS) were measured before, 5 min, 24 h, 48 h, and 72 h after the test. The sleep/wake cycle was monitored during the night after ingestion. Results: Data were analysed using paired t-tests, Wilcoxon tests, and two-way ANOVAs, with significance set at <i>p</i> < 0.05. Compared to PLA, MEL did not modify any sleep parameters or blood markers (all <i>p</i> > 0.05). However, MEL improved total distance, fatigue index, the percentage decrement between sprints, and HR<sub>peak</sub> (all <i>p</i> < 0.05) in the 5mSRT compared to PLA. MEL also enhanced PRS values up to 72 h post-exercise and reduced DOMS (all <i>p</i> < 0.05). Conclusion: In summary, 6 mg of melatonin taken at night enhanced next-day high-intensity exercise performance and improved perceived recovery up to 72 h post-exercise.https://www.mdpi.com/2075-4663/13/6/190ergogenic aidmuscle damageinflammationrecoverysleepsports performance
spellingShingle Nourhène Mahdi
Slaheddine Delleli
Arwa Jebabli
Khouloud Ben Maaoui
Juan Del Coso
Hamdi Chtourou
Luca Paolo Ardigò
Ibrahim Ouergui
Melatonin Supplementation Enhances Next-Day High-Intensity Exercise Performance and Recovery in Trained Males: A Placebo-Controlled Crossover Study
Sports
ergogenic aid
muscle damage
inflammation
recovery
sleep
sports performance
title Melatonin Supplementation Enhances Next-Day High-Intensity Exercise Performance and Recovery in Trained Males: A Placebo-Controlled Crossover Study
title_full Melatonin Supplementation Enhances Next-Day High-Intensity Exercise Performance and Recovery in Trained Males: A Placebo-Controlled Crossover Study
title_fullStr Melatonin Supplementation Enhances Next-Day High-Intensity Exercise Performance and Recovery in Trained Males: A Placebo-Controlled Crossover Study
title_full_unstemmed Melatonin Supplementation Enhances Next-Day High-Intensity Exercise Performance and Recovery in Trained Males: A Placebo-Controlled Crossover Study
title_short Melatonin Supplementation Enhances Next-Day High-Intensity Exercise Performance and Recovery in Trained Males: A Placebo-Controlled Crossover Study
title_sort melatonin supplementation enhances next day high intensity exercise performance and recovery in trained males a placebo controlled crossover study
topic ergogenic aid
muscle damage
inflammation
recovery
sleep
sports performance
url https://www.mdpi.com/2075-4663/13/6/190
work_keys_str_mv AT nourhenemahdi melatoninsupplementationenhancesnextdayhighintensityexerciseperformanceandrecoveryintrainedmalesaplacebocontrolledcrossoverstudy
AT slaheddinedelleli melatoninsupplementationenhancesnextdayhighintensityexerciseperformanceandrecoveryintrainedmalesaplacebocontrolledcrossoverstudy
AT arwajebabli melatoninsupplementationenhancesnextdayhighintensityexerciseperformanceandrecoveryintrainedmalesaplacebocontrolledcrossoverstudy
AT khouloudbenmaaoui melatoninsupplementationenhancesnextdayhighintensityexerciseperformanceandrecoveryintrainedmalesaplacebocontrolledcrossoverstudy
AT juandelcoso melatoninsupplementationenhancesnextdayhighintensityexerciseperformanceandrecoveryintrainedmalesaplacebocontrolledcrossoverstudy
AT hamdichtourou melatoninsupplementationenhancesnextdayhighintensityexerciseperformanceandrecoveryintrainedmalesaplacebocontrolledcrossoverstudy
AT lucapaoloardigo melatoninsupplementationenhancesnextdayhighintensityexerciseperformanceandrecoveryintrainedmalesaplacebocontrolledcrossoverstudy
AT ibrahimouergui melatoninsupplementationenhancesnextdayhighintensityexerciseperformanceandrecoveryintrainedmalesaplacebocontrolledcrossoverstudy