Droplet digital RT-PCR method for SARS-CoV-2 variants detection in clinical and wastewater samples

Droplet digital RT-PCR method for SARS-CoV-2 variants detection in clinical and wastewater samples

ObjectiveTo establish a sensitive, specific, and precise quantitative detection method for SARS-CoV-2 variants using droplet digital RT-PCR (RT-ddPCR).MethodsDual primer-probe sets targeting the SARS-CoV-2 nucleocapsid (N) and spike (S) genes were designed. The annealing temperature for RT-ddPCR was...

Full description

Saved in:
Bibliographic Details
Main Authors: Feng Wang, Yi Sun, Liming Gong, Lingxuan Su, Biaofeng Zhou, Xiuyu Lou, Yin Chen, Wen Shi, Haiyan Mao, Yanjun Zhang
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-07-01
Series:Frontiers in Microbiology
Subjects:
droplet digital RT-PCR
SARS-CoV-2 variants
clinical
wastewater
sensitivity
specificity
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2025.1635733/full
Tags: Add Tag
No Tags, Be the first to tag this record!

Internet

https://www.frontiersin.org/articles/10.3389/fmicb.2025.1635733/full

Similar Items