Quality by design for transient RBD-Fc fusion protein production in Chinese hamster ovary cells

Quality by design (QbD) is applied to the upstream process to maximize the RBD-Fc fusion protein production in CHO cells. The three factors (culture duration, temperature, and polyethyleneimine to plasmid DNA (PEI-Max/pDNA) ratio) were identified as critical process attributes based on risk analysis...

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Bibliographic Details
Main Authors: Araya Jivapetthai, Wanatchaporn Arunmanee, Natapol Pornputtapong
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:Biotechnology Reports
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2215017X25000098
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