A protocol for immunostaining of macrophages in whole-mount mouse cornea, conjunctiva, and lacrimal gland

Summary: The healthy lacrimal functional unit contains a resident macrophage population. Here, we present a protocol for immunofluorescent staining of macrophage markers, CD11b, F4/80, and CD206, in whole-mount mouse cornea, conjunctiva, and 50-μM-thick lacrimal gland section. We describe steps for...

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Bibliographic Details
Main Authors: Saleh Alfuraih, Dhruv Patel, Wonsuk Choi, Karthikeyan Ramasamy, Rais Ansari, Ajay Sharma
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:STAR Protocols
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724006099
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