Targeted imaging of pulmonary fibrosis by a cyclic peptide LyP-1

Targeted imaging of pulmonary fibrosis by a cyclic peptide LyP-1

Abstract Pulmonary fibrosis (PF) is an interstitial chronic lung disease characterized by interstitial inflammation and extracellular matrix deposition, resulting in progressive lung dysfunction and ultimate respiratory failure. However, lacking of precise and noninvasive tracers for fibrotic lesion...

Full description

Saved in:
Bibliographic Details
Main Authors: Jing Li, Rui Shu, Tian Peng, Zifeng Yang, Mingzi Yang, Fengjiao Hu, Zhangqian Tao, Ying Hong, Zhiwei Cai, Jing Jia, Lu Wan, Song Tian, Zhi-Gang She, Hongliang Li, Xiao-Jing Zhang, Ejuan Zhang
Format: Article
Language:English
Published: Nature Portfolio 2025-03-01
Series:Scientific Reports
Subjects:
Pulmonary fibrosis
LyP-1
Positron emission tomography
Tracer
Peptide
Non-invasive imaging
Online Access:https://doi.org/10.1038/s41598-024-78068-0
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Pulmonary fibrosis (PF) is an interstitial chronic lung disease characterized by interstitial inflammation and extracellular matrix deposition, resulting in progressive lung dysfunction and ultimate respiratory failure. However, lacking of precise and noninvasive tracers for fibrotic lesions limits timely diagnosis and treatment. Here, we identified LyP-1, a cyclic peptide, as a specific and sensitive tracer for PF detection using PET/CT imaging. FITC-LyP-1 selectively recognized fibrotic regions in bleomycin-induced PF mice, indicating its targeting capability. The colocalization of FITC-LyP-1 with extracellular collagen I within the fibrotic lesions validated its specificity, and further analysis revealed several potential target molecules. In the in vivo application studies, radiolabeled [68Ga]Ga-LyP-1 showed significantly increased lung uptake in PF mice, specifically enriching fibrotic regions on PET/CT imaging. Notably, compared to CT imaging that showed increased mean lung density throughout the phases after bleomycin-administration, lung uptake of [68Ga]Ga-LyP-1 was only increased in the later phase, indicating that LyP-1 recognizes the fibrous changes rather than the inflammatory cells in vivo. These results suggest that the new radiotracer [68Ga]Ga-LyP-1 specifically detects the extracellular matrix in fibrotic lungs. LyP-1 shows promise as a noninvasive tracer for assessing human pulmonary fibrosis, offering potential for improved diagnostic accuracy and timely intervention.
ISSN:2045-2322

Similar Items