Protective effect of marine animal hydrolysis-peptides on adjuvant-induced arthritis in mice by gut microbiota and short-chain fatty acids

Marine fauna provides a plentiful repository of peptides and bioactive proteins. Peptides and proteins isolated from marine animals have been studied and applied in the development of food supplements, drugs, and cosmeceutical products because of their special bioactivities, such as anti-inflammator...

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Main Authors: Zhen Zhang, Shasha Tang, Ziyan Wang, Jiaojiao Han, Jun Zhou, Chenyang Lu, Bin Liu, Xiaoling Sun, Rongxian Yu, Rixin Wang, Xiurong Su
Format: Article
Language:English
Published: Tsinghua University Press 2025-01-01
Series:Food Science and Human Wellness
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Online Access:https://www.sciopen.com/article/10.26599/FSHW.2024.9250089
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author Zhen Zhang
Shasha Tang
Ziyan Wang
Jiaojiao Han
Jun Zhou
Chenyang Lu
Bin Liu
Xiaoling Sun
Rongxian Yu
Rixin Wang
Xiurong Su
author_facet Zhen Zhang
Shasha Tang
Ziyan Wang
Jiaojiao Han
Jun Zhou
Chenyang Lu
Bin Liu
Xiaoling Sun
Rongxian Yu
Rixin Wang
Xiurong Su
author_sort Zhen Zhang
collection DOAJ
description Marine fauna provides a plentiful repository of peptides and bioactive proteins. Peptides and proteins isolated from marine animals have been studied and applied in the development of food supplements, drugs, and cosmeceutical products because of their special bioactivities, such as anti-inflammatory and antioxidant effects. This study focused on exploring the alleviating effects of five major marine animal-derived peptides (Apostichopus japonicus, Acaudina leucoprocta, Melanogrammus aeglefinus, Phascolosoma esculenta and Rhopilema esculentum) on adjuvant-induced arthritis (AIA). The treatment with five marine animals-derived peptides downregulated the expression levels of pro-inflammatory cytokines of interleukin (IL)-1β, IL-17 and tumor necrosis factor (TNF)-α in the bones of the mice with AIA and alleviated the rough surface of bone tissues significantly. A. japonicus-treatment ameliorates inflammation by restoring nuclear factor-κB pathway in AIA mice. High-throughput sequencing of the gut microbiota based on 16S rRNA sequencing revealed that A. japonicus peptide-treated AIA mice showed alterations and imbalance of intestinal flora and an increased abundance of Lactobacillus and Clostridium. Furthermore, metabolomic analysis showed that the level of short-chain fatty acids (SCFAs) in the feces was enhanced to different degrees in mice treated with five major marine animal-derived peptides. Taken together, we propose that major marine animal-derived peptides can alleviate arthritis by improving the imbalance in the gut flora and increasing SCFAs production to varying degrees.
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spelling doaj-art-d32c8c2c22514b0ea8dc2357178e23882025-08-20T02:05:07ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302025-01-01141925008910.26599/FSHW.2024.9250089Protective effect of marine animal hydrolysis-peptides on adjuvant-induced arthritis in mice by gut microbiota and short-chain fatty acidsZhen Zhang0Shasha Tang1Ziyan Wang2Jiaojiao Han3Jun Zhou4Chenyang Lu5Bin Liu6Xiaoling Sun7Rongxian Yu8Rixin Wang9Xiurong Su10State Key Laboratory of Agricultural Products Safety, Ningbo University, Ningbo 315211, ChinaState Key Laboratory of Agricultural Products Safety, Ningbo University, Ningbo 315211, ChinaState Key Laboratory of Agricultural Products Safety, Ningbo University, Ningbo 315211, ChinaState Key Laboratory of Agricultural Products Safety, Ningbo University, Ningbo 315211, ChinaState Key Laboratory of Agricultural Products Safety, Ningbo University, Ningbo 315211, ChinaState Key Laboratory of Agricultural Products Safety, Ningbo University, Ningbo 315211, ChinaVigor Technology Group, Shenzhen 518118, ChinaVigor Technology Group, Shenzhen 518118, ChinaVigor Technology Group, Shenzhen 518118, ChinaSchool of Marine Science, Ningbo University, Ningbo 315832, ChinaState Key Laboratory of Agricultural Products Safety, Ningbo University, Ningbo 315211, ChinaMarine fauna provides a plentiful repository of peptides and bioactive proteins. Peptides and proteins isolated from marine animals have been studied and applied in the development of food supplements, drugs, and cosmeceutical products because of their special bioactivities, such as anti-inflammatory and antioxidant effects. This study focused on exploring the alleviating effects of five major marine animal-derived peptides (Apostichopus japonicus, Acaudina leucoprocta, Melanogrammus aeglefinus, Phascolosoma esculenta and Rhopilema esculentum) on adjuvant-induced arthritis (AIA). The treatment with five marine animals-derived peptides downregulated the expression levels of pro-inflammatory cytokines of interleukin (IL)-1β, IL-17 and tumor necrosis factor (TNF)-α in the bones of the mice with AIA and alleviated the rough surface of bone tissues significantly. A. japonicus-treatment ameliorates inflammation by restoring nuclear factor-κB pathway in AIA mice. High-throughput sequencing of the gut microbiota based on 16S rRNA sequencing revealed that A. japonicus peptide-treated AIA mice showed alterations and imbalance of intestinal flora and an increased abundance of Lactobacillus and Clostridium. Furthermore, metabolomic analysis showed that the level of short-chain fatty acids (SCFAs) in the feces was enhanced to different degrees in mice treated with five major marine animal-derived peptides. Taken together, we propose that major marine animal-derived peptides can alleviate arthritis by improving the imbalance in the gut flora and increasing SCFAs production to varying degrees.https://www.sciopen.com/article/10.26599/FSHW.2024.9250089marine animals-derived peptidesadjuvant-induced arthritisgut florashort-chain fatty acid
spellingShingle Zhen Zhang
Shasha Tang
Ziyan Wang
Jiaojiao Han
Jun Zhou
Chenyang Lu
Bin Liu
Xiaoling Sun
Rongxian Yu
Rixin Wang
Xiurong Su
Protective effect of marine animal hydrolysis-peptides on adjuvant-induced arthritis in mice by gut microbiota and short-chain fatty acids
Food Science and Human Wellness
marine animals-derived peptides
adjuvant-induced arthritis
gut flora
short-chain fatty acid
title Protective effect of marine animal hydrolysis-peptides on adjuvant-induced arthritis in mice by gut microbiota and short-chain fatty acids
title_full Protective effect of marine animal hydrolysis-peptides on adjuvant-induced arthritis in mice by gut microbiota and short-chain fatty acids
title_fullStr Protective effect of marine animal hydrolysis-peptides on adjuvant-induced arthritis in mice by gut microbiota and short-chain fatty acids
title_full_unstemmed Protective effect of marine animal hydrolysis-peptides on adjuvant-induced arthritis in mice by gut microbiota and short-chain fatty acids
title_short Protective effect of marine animal hydrolysis-peptides on adjuvant-induced arthritis in mice by gut microbiota and short-chain fatty acids
title_sort protective effect of marine animal hydrolysis peptides on adjuvant induced arthritis in mice by gut microbiota and short chain fatty acids
topic marine animals-derived peptides
adjuvant-induced arthritis
gut flora
short-chain fatty acid
url https://www.sciopen.com/article/10.26599/FSHW.2024.9250089
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