Cytoplasmic RNA Extraction from Fresh and Frozen Mammalian Tissues
The quality of collections of expressed sequence tags and full-length cDNAs is adversely affected by the presence of “junk” clones derived from unspliced or partially spliced RNAs present in conventional total RNA preparations. One can overcome this problem by using intact cytoplasmic RNA to create...
Saved in:
| Main Authors: | P. Carninci, M. Nakamura, K. Sato, Y. Hayashizaki, M.J. Brownstein |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2002-08-01
|
| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/02332st01 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Detection of fusion events by RNA sequencing in FFPE versus freshly frozen colorectal cancer tissue samples
by: Maxim Sorokin, et al.
Published: (2025-01-01) -
Cytoplasmic injection of circular plasmids allows targeted expression in mammalian embryos
by: Khursheed Iqbal, et al.
Published: (2009-11-01) -
Homogenization of Mammalian Tissues
by: John Graham
Published: (2002-01-01) -
Cryogenic mouse tissue homogenization as an alternative to fresh-frozen biopsy use for genomics, transcriptomics, proteomics and metabolomics
by: Laimdota Zizmare, et al.
Published: (2025-06-01) -
Comparison of the miRNA expression profiles in fresh frozen and formalin-fixed paraffin-embedded tonsillar tumors.
by: Zuzana Vojtechova, et al.
Published: (2017-01-01)