Cytoplasmic RNA Extraction from Fresh and Frozen Mammalian Tissues

The quality of collections of expressed sequence tags and full-length cDNAs is adversely affected by the presence of “junk” clones derived from unspliced or partially spliced RNAs present in conventional total RNA preparations. One can overcome this problem by using intact cytoplasmic RNA to create...

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Bibliographic Details
Main Authors: P. Carninci, M. Nakamura, K. Sato, Y. Hayashizaki, M.J. Brownstein
Format: Article
Language:English
Published: Taylor & Francis Group 2002-08-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/02332st01
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