Iterative crRNA design and a PAM-free strategy enabled an ultra-specific RPA-CRISPR/Cas12a detection platform

Iterative crRNA design and a PAM-free strategy enabled an ultra-specific RPA-CRISPR/Cas12a detection platform

Abstract CRISPR/Cas12a is a highly promising detection tool. However, detecting single nucleotide variations (SNVs) remains challenging. Here, we elucidate Cas12a specificity through crRNA engineering and profiling of single- and double-base mismatch tolerance across three targets. Our findings indi...

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Main Authors: Xujian Mao, Jian Xu, Jingyi Jiang, Qiong Li, Ping Yao, Jinyi Jiang, Li Gong, Yin Dong, Bowen Tu, Rong Wang, Hongbing Tang, Fang Yao, Fengming Wang
Format: Article
Language:English
Published: Nature Portfolio 2024-11-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-024-07173-7
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https://doi.org/10.1038/s42003-024-07173-7

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