pH-FISH: coupled microscale analysis of microbial identity and acid–base metabolism in complex biofilm samples

pH-FISH: coupled microscale analysis of microbial identity and acid–base metabolism in complex biofilm samples

Abstract Background Correlative structural and chemical imaging of biofilms allows for the combined analysis of microbial identity and metabolism at the microscale. Here, we developed pH-FISH, a method that combines pH ratiometry with fluorescence in situ hybridization (FISH) in structurally intact...

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Bibliographic Details
Main Authors: Yumi Chokyu Del Rey, Katharina Kitzinger, Marie Braad Lund, Andreas Schramm, Rikke Louise Meyer, Michael Wagner, Sebastian Schlafer
Format: Article
Language:English
Published: BMC 2024-12-01
Series:Microbiome
Subjects:
Fluorescence in situ hybridization
pH ratiometry
Confocal laser scanning microscopy
Microenvironments
Biofilms
Dental biofilms
Online Access:https://doi.org/10.1186/s40168-024-01977-9
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https://doi.org/10.1186/s40168-024-01977-9

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