CoreTIA: a modular, statistically robust transduction inhibition assay for AAV neutralization

CoreTIA: a modular, statistically robust transduction inhibition assay for AAV neutralization

Adeno-associated virus (AAV) gene therapy is often limited by pre-existing neutralizing antibodies (NAbs), yet current assays for NAb detection lack standardization and rarely quantify uncertainty, complicating cross-study comparisons. We present coreTIA (core Transduction Inhibition Assay), a compr...

Full description

Saved in:

Bibliographic Details
Main Authors:	Beatrix Kovács, Fanni Somogyi, Viktória Szabó, Zoltán Zsolt Nagy, István Hernádi, Ferenc Mátyás, Wim Vanduffel, Zsuzsanna Szemlaky, Balázs Rózsa, István Ulbert, Dániel Hillier
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2025-08-01
Series:	Frontiers in Immunology
Subjects:	AAV neutralizing antibodies gene therapy immunogenicity neutralizing antibody titer Bayesian dose-response modeling ND50 quantification with uncertainty AAV serotype optimization
Online Access:	https://www.frontiersin.org/articles/10.3389/fimmu.2025.1623848/full
Tags:	Add Tag No Tags, Be the first to tag this record!

Similar Items

Distinct infectivity and neutralization antibody responses in the highly homologous AAV Go.1 and AAV5
by: Mei Li, et al.
Published: (2025-04-01)

Retinal Epithelial Neutralization Assay Optimizes AAV Serotype Selection for Ocular Gene Therapy
by: Yao Li, et al.
Published: (2025-07-01)

iPSC-hepatocyte organoids as a novel platform to predict AAV gene therapy efficacy
by: Estelle Berreur, et al.
Published: (2025-06-01)

Assessment of the Level of Virus Neutralizing Antibodies to Enterovirus 71 in Children in Ekaterinburg (2010–2017)
by: O. M. Olenkova, et al.
Published: (2019-05-01)

Common AAV gene therapy vectors show nonselective transduction of ex vivo human brain tissue
by: JP McGinnis, et al.
Published: (2025-06-01)

Evaluation of the loading capacity and patterns of packaged DNA in AAV genomes of different sizes using long-read sequencing
by: Mitsuki Kosaka, et al.
Published: (2025-06-01)

Self-silencing adenovirus enables precise infectious titration of recombinant adeno-associated viral vectors
by: Chloé Fustinoni, et al.
Published: (2025-06-01)

AAV-mediated transduction of songbird retina
by: Pranav Kumar Seth, et al.
Published: (2025-03-01)

Genetic Variability Related Behavioral Plasticity in Pikeperch (<i>Sander lucioperca</i> L.) Fingerlings
by: Ildikó Benedek, et al.
Published: (2025-07-01)

AAV‐mediated Gene Therapy for Hereditary Deafness: Progress and Perspectives
by: Liyan Zhang, et al.
Published: (2024-12-01)

A comprehensive study of AAV tropism across C57BL/6 mice, BALB/c mice, and crab-eating macaques
by: Kailun Fang, et al.
Published: (2025-03-01)

αFAP-specific nanobodies mediate a highly precise retargeting of modified AAV2 capsids thereby enabling specific transduction of tumor tissues
by: Olaniyi Olarewaju, et al.
Published: (2024-12-01)

Fusion of Visual Attention and Scene Descriptions With Deep Reinforcement Learning for AAV Indoor Autonomous Navigation
by: Hussein Samma, et al.
Published: (2025-01-01)

Novel AAV capsids for intravitreal gene therapy of photoreceptor disorders
by: Marina Pavlou, et al.
Published: (2021-02-01)

Comparative analysis of AAV serotypes for transduction of olfactory sensory neurons
by: Benjamin D. W. Belfort, et al.
Published: (2025-02-01)

Reversing Pathology in an Aggravated Fabry Mouse Model Using Low-Dose Engineered Human Alpha-Galactosidase A AAV Gene Therapy
by: Wanida Ruangsiriluk, et al.
Published: (2025-02-01)

Intention Recognition of AAV Swarm Based on GAT-EPool-BiGRU Model
by: Jiajun Yuan, et al.
Published: (2025-01-01)

Editorial: The immune response to therapeutic antibodies
by: Michael G. Tovey, et al.
Published: (2025-02-01)

AAV induces hepatic necroptosis and carcinoma in diabetic and obese mice dependent on Pebp1 pathway
by: Yalan Cheng, et al.
Published: (2023-06-01)

Long-term inhibition of Hepatitis B virus gene expression by a primary microrna expressing ancestral adeno-associated viral vector
by: Njabulo Ziphezinhle Mnyandu, et al.
Published: (2025-02-01)

Pre-existing Anti-AAV9 antibodies in the Chinese healthy and rare disease populations: Implications for gene therapy
by: Qian Zhao, et al.
Published: (2025-04-01)

AAV-2 VECTOR INTEGRATE INTO THE OVINE MYOBLAST GENOME RANDOMLY AND PROMOTE DIFFERENTIATION AND PROLIFERATION VIA FOLLISTATIN OVER-EXPRESSION OF ERK1/2 AND AKT SIGNALING PATHWAYS
by: Mahmood Nazari, et al.
Published: (2016-11-01)

Secreted PD-L1 alleviates inflammatory arthritis in mice through local and systemic AAV gene therapy
by: Wenjun Li, et al.
Published: (2025-02-01)

AAVR Expression is Essential for AAV Vector Transduction in Sensory Hair Cells
by: Fan Wu, et al.
Published: (2025-08-01)

Advances in AAV-mediated gene replacement therapy for pediatric monogenic neurological disorders
by: Livia Zhou, et al.
Published: (2024-12-01)

Development of circular AAV cargos for targeted seamless insertion with large serine integrases
by: Brett J.G. Estes, et al.
Published: (2025-06-01)

Artificial Intelligence‐Based Approaches for AAV Vector Engineering
by: Fangzhi Tan, et al.
Published: (2025-03-01)

Long-Term Immune Consequences of Initial SARS-CoV-2 A.23.1 Exposure: A Longitudinal Study of Antibody Responses and Cross-Neutralization in a Ugandan Cohort
by: Gerald Kevin Oluka, et al.
Published: (2025-01-01)

Optimization of Communication Quality for Energy-Limited Inspection AAV: A Hybrid Algorithm
by: Wei Wang, et al.
Published: (2024-01-01)

AAV-mediated expression of proneural factors stimulates neurogenesis from adult Müller glia in vivo
by: Marina Pavlou, et al.
Published: (2025-03-01)

AAV‐mediated delivery of an anti‐BACE1 VHH alleviates pathology in an Alzheimer's disease model
by: Marika Marino, et al.
Published: (2022-03-01)

Comparative Studies of Descriptor-Based Image Matching Techniques for AAV Applications
by: Tomasz Pogorzelski, et al.
Published: (2025-01-01)

The Effect of Processing Techniques on the Classification Accuracy of Brain-Computer Interface Systems
by: András Adolf, et al.
Published: (2024-12-01)

Development of cell-based assay for detecting replication-competent adeno-associated virus by qPCR
by: Aishwarya Shevade, et al.
Published: (2025-09-01)

Longevity of antibody responses is associated with distinct antigen-specific B cell subsets early after infection
by: Lisan H. Kuijper, et al.
Published: (2024-12-01)

Effective delivery of large genes to the retina by dual AAV vectors
by: Ivana Trapani, et al.
Published: (2013-12-01)

Identification of the role of SNARE proteins in rAAV vector production through interaction with the viral MAAP
by: Cagla Aksu Kuz, et al.
Published: (2025-03-01)

Evaluating the Protective Role of Intranasally Administered Avian-Derived IgY Against SARS-CoV-2 in Syrian Hamster Models
by: Mónika Madai, et al.
Published: (2024-12-01)

Characterization of factors that influence rAAV yield and quality when produced using rHSV co-infection
by: Xiaofei E, et al.
Published: (2025-06-01)

A novel platform for engineered AAV-based vaccines
by: Sabrina Babutzka, et al.
Published: (2025-03-01)