EFFICIENT RIBOSOMAL RNA DEPLETION FROM DROSOPHILA TOTAL RNA FOR NEXT-GENERATION SEQUENCING APPLICATIONS [version 2; peer review: 2 approved]

We developed a cost-effective enzyme-based rRNA-depletion method tailored for Drosophila melanogaster, addressing the limitations of existing commercial kits and the lack of peer-reviewed alternatives. Our method employs single-stranded DNA probes complementary to Drosophila rRNA, forming DNA-RNA hy...

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Bibliographic Details
Main Authors: Baskar Bakthavachalu, Awadhesh Pandit, Omkar Koppaka, Ankita Chodankar, Shweta Tandon
Format: Article
Language:English
Published: Wellcome 2025-05-01
Series:Wellcome Open Research
Subjects:
Online Access:https://wellcomeopenresearch.org/articles/10-112/v2
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