Improved porosity promotes reendothelialization and smooth muscle remodeling in decellularized tissue-engineered vascular grafts
Decellularized tissue-engineered vascular grafts (dTEVGs) exhibit superior biocompatibility, anti-infection properties and repair potential, contributing to better patency and making them a more ideal choice for arteriovenous grafts (AVGs) in hemodialysis compared to chemically synthesized grafts. H...
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Elsevier
2025-02-01
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author | Tun Wang Sheng Liao Peng Lu Zhenyu He Siyuan Cheng Tianjian Wang Zibo Cheng Yangyang An Mo Wang Chang Shu |
author_facet | Tun Wang Sheng Liao Peng Lu Zhenyu He Siyuan Cheng Tianjian Wang Zibo Cheng Yangyang An Mo Wang Chang Shu |
author_sort | Tun Wang |
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description | Decellularized tissue-engineered vascular grafts (dTEVGs) exhibit superior biocompatibility, anti-infection properties and repair potential, contributing to better patency and making them a more ideal choice for arteriovenous grafts (AVGs) in hemodialysis compared to chemically synthesized grafts. However, the unsatisfactory reendothelialization and smooth muscle remodeling of current dTEVGs limit their advantages. In this study, we investigated the use of elastase to improve the porosity of elastic fiber layers in dTEVGs, aiming to promote cell infiltration and achieve superior reendothelialization and smooth muscle remodeling. Our findings revealed that elastase treatment induced scattered cracks and holes in the elastic fiber layers of dTEVGs. Porous dTEVGs demonstrated increased cell infiltration in rat subcutaneous tissue. In the rat AVG models, mildly elastase-treated dTEVGs significantly improved cell infiltration and graft remodeling, including adequate smooth muscle cell (SMC) repopulation, impressive reendothelization and regeneration of the extracellular matrix, without stenosis, dilation or disintegration of the grafts. This study demonstrates that porous dTEVGs promote reendothelization, smooth muscle remodeling and extracellular matrix regeneration while retaining a stable graft structure, enhancing durability and puncture resistance in hemodialysis. |
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language | English |
publishDate | 2025-02-01 |
publisher | Elsevier |
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spelling | doaj-art-d051f236605c4df4a7c92d8f91aa54152025-01-17T04:52:06ZengElsevierMaterials Today Bio2590-00642025-02-0130101402Improved porosity promotes reendothelialization and smooth muscle remodeling in decellularized tissue-engineered vascular graftsTun Wang0Sheng Liao1Peng Lu2Zhenyu He3Siyuan Cheng4Tianjian Wang5Zibo Cheng6Yangyang An7Mo Wang8Chang Shu9Department of Vascular Surgery, The Second Xiangya Hospital, Central South University, Changsha, 410011, China; Institute of Vascular Diseases, Central South University, Changsha, 410011, ChinaDepartment of Vascular Surgery, The Second Xiangya Hospital, Central South University, Changsha, 410011, China; Institute of Vascular Diseases, Central South University, Changsha, 410011, ChinaDepartment of Vascular Surgery, The Second Xiangya Hospital, Central South University, Changsha, 410011, China; Institute of Vascular Diseases, Central South University, Changsha, 410011, ChinaDepartment of Vascular Surgery, The Second Xiangya Hospital, Central South University, Changsha, 410011, China; Institute of Vascular Diseases, Central South University, Changsha, 410011, ChinaDepartment of Vascular Surgery, The Second Xiangya Hospital, Central South University, Changsha, 410011, China; Institute of Vascular Diseases, Central South University, Changsha, 410011, ChinaDepartment of Vascular Surgery, The Second Xiangya Hospital, Central South University, Changsha, 410011, China; Institute of Vascular Diseases, Central South University, Changsha, 410011, ChinaDepartment of Vascular Surgery, The Second Xiangya Hospital, Central South University, Changsha, 410011, China; Institute of Vascular Diseases, Central South University, Changsha, 410011, ChinaDepartment of Vascular Surgery, The Second Xiangya Hospital, Central South University, Changsha, 410011, China; Institute of Vascular Diseases, Central South University, Changsha, 410011, ChinaDepartment of Vascular Surgery, The Second Xiangya Hospital, Central South University, Changsha, 410011, China; Institute of Vascular Diseases, Central South University, Changsha, 410011, ChinaDepartment of Vascular Surgery, The Second Xiangya Hospital, Central South University, Changsha, 410011, China; Institute of Vascular Diseases, Central South University, Changsha, 410011, China; Center of Vascular Surgery, Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100037, China; Corresponding author. Department of Vascular Surgery, The Second Xiangya Hospital, Central South University, Changsha 410011, China. Institute of Vascular Diseases, Central South University, Changsha 410011, China. Center of Vascular Surgery, Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China.Decellularized tissue-engineered vascular grafts (dTEVGs) exhibit superior biocompatibility, anti-infection properties and repair potential, contributing to better patency and making them a more ideal choice for arteriovenous grafts (AVGs) in hemodialysis compared to chemically synthesized grafts. However, the unsatisfactory reendothelialization and smooth muscle remodeling of current dTEVGs limit their advantages. In this study, we investigated the use of elastase to improve the porosity of elastic fiber layers in dTEVGs, aiming to promote cell infiltration and achieve superior reendothelialization and smooth muscle remodeling. Our findings revealed that elastase treatment induced scattered cracks and holes in the elastic fiber layers of dTEVGs. Porous dTEVGs demonstrated increased cell infiltration in rat subcutaneous tissue. In the rat AVG models, mildly elastase-treated dTEVGs significantly improved cell infiltration and graft remodeling, including adequate smooth muscle cell (SMC) repopulation, impressive reendothelization and regeneration of the extracellular matrix, without stenosis, dilation or disintegration of the grafts. This study demonstrates that porous dTEVGs promote reendothelization, smooth muscle remodeling and extracellular matrix regeneration while retaining a stable graft structure, enhancing durability and puncture resistance in hemodialysis.http://www.sciencedirect.com/science/article/pii/S2590006424004630Tissue-engineered vascular graftReendothelializationRemodelingArteriovenous graftHemodialysis |
spellingShingle | Tun Wang Sheng Liao Peng Lu Zhenyu He Siyuan Cheng Tianjian Wang Zibo Cheng Yangyang An Mo Wang Chang Shu Improved porosity promotes reendothelialization and smooth muscle remodeling in decellularized tissue-engineered vascular grafts Materials Today Bio Tissue-engineered vascular graft Reendothelialization Remodeling Arteriovenous graft Hemodialysis |
title | Improved porosity promotes reendothelialization and smooth muscle remodeling in decellularized tissue-engineered vascular grafts |
title_full | Improved porosity promotes reendothelialization and smooth muscle remodeling in decellularized tissue-engineered vascular grafts |
title_fullStr | Improved porosity promotes reendothelialization and smooth muscle remodeling in decellularized tissue-engineered vascular grafts |
title_full_unstemmed | Improved porosity promotes reendothelialization and smooth muscle remodeling in decellularized tissue-engineered vascular grafts |
title_short | Improved porosity promotes reendothelialization and smooth muscle remodeling in decellularized tissue-engineered vascular grafts |
title_sort | improved porosity promotes reendothelialization and smooth muscle remodeling in decellularized tissue engineered vascular grafts |
topic | Tissue-engineered vascular graft Reendothelialization Remodeling Arteriovenous graft Hemodialysis |
url | http://www.sciencedirect.com/science/article/pii/S2590006424004630 |
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