Improved porosity promotes reendothelialization and smooth muscle remodeling in decellularized tissue-engineered vascular grafts

Decellularized tissue-engineered vascular grafts (dTEVGs) exhibit superior biocompatibility, anti-infection properties and repair potential, contributing to better patency and making them a more ideal choice for arteriovenous grafts (AVGs) in hemodialysis compared to chemically synthesized grafts. H...

Full description

Saved in:
Bibliographic Details
Main Authors: Tun Wang, Sheng Liao, Peng Lu, Zhenyu He, Siyuan Cheng, Tianjian Wang, Zibo Cheng, Yangyang An, Mo Wang, Chang Shu
Format: Article
Language:English
Published: Elsevier 2025-02-01
Series:Materials Today Bio
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2590006424004630
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1841533338502823936
author Tun Wang
Sheng Liao
Peng Lu
Zhenyu He
Siyuan Cheng
Tianjian Wang
Zibo Cheng
Yangyang An
Mo Wang
Chang Shu
author_facet Tun Wang
Sheng Liao
Peng Lu
Zhenyu He
Siyuan Cheng
Tianjian Wang
Zibo Cheng
Yangyang An
Mo Wang
Chang Shu
author_sort Tun Wang
collection DOAJ
description Decellularized tissue-engineered vascular grafts (dTEVGs) exhibit superior biocompatibility, anti-infection properties and repair potential, contributing to better patency and making them a more ideal choice for arteriovenous grafts (AVGs) in hemodialysis compared to chemically synthesized grafts. However, the unsatisfactory reendothelialization and smooth muscle remodeling of current dTEVGs limit their advantages. In this study, we investigated the use of elastase to improve the porosity of elastic fiber layers in dTEVGs, aiming to promote cell infiltration and achieve superior reendothelialization and smooth muscle remodeling. Our findings revealed that elastase treatment induced scattered cracks and holes in the elastic fiber layers of dTEVGs. Porous dTEVGs demonstrated increased cell infiltration in rat subcutaneous tissue. In the rat AVG models, mildly elastase-treated dTEVGs significantly improved cell infiltration and graft remodeling, including adequate smooth muscle cell (SMC) repopulation, impressive reendothelization and regeneration of the extracellular matrix, without stenosis, dilation or disintegration of the grafts. This study demonstrates that porous dTEVGs promote reendothelization, smooth muscle remodeling and extracellular matrix regeneration while retaining a stable graft structure, enhancing durability and puncture resistance in hemodialysis.
format Article
id doaj-art-d051f236605c4df4a7c92d8f91aa5415
institution Kabale University
issn 2590-0064
language English
publishDate 2025-02-01
publisher Elsevier
record_format Article
series Materials Today Bio
spelling doaj-art-d051f236605c4df4a7c92d8f91aa54152025-01-17T04:52:06ZengElsevierMaterials Today Bio2590-00642025-02-0130101402Improved porosity promotes reendothelialization and smooth muscle remodeling in decellularized tissue-engineered vascular graftsTun Wang0Sheng Liao1Peng Lu2Zhenyu He3Siyuan Cheng4Tianjian Wang5Zibo Cheng6Yangyang An7Mo Wang8Chang Shu9Department of Vascular Surgery, The Second Xiangya Hospital, Central South University, Changsha, 410011, China; Institute of Vascular Diseases, Central South University, Changsha, 410011, ChinaDepartment of Vascular Surgery, The Second Xiangya Hospital, Central South University, Changsha, 410011, China; Institute of Vascular Diseases, Central South University, Changsha, 410011, ChinaDepartment of Vascular Surgery, The Second Xiangya Hospital, Central South University, Changsha, 410011, China; Institute of Vascular Diseases, Central South University, Changsha, 410011, ChinaDepartment of Vascular Surgery, The Second Xiangya Hospital, Central South University, Changsha, 410011, China; Institute of Vascular Diseases, Central South University, Changsha, 410011, ChinaDepartment of Vascular Surgery, The Second Xiangya Hospital, Central South University, Changsha, 410011, China; Institute of Vascular Diseases, Central South University, Changsha, 410011, ChinaDepartment of Vascular Surgery, The Second Xiangya Hospital, Central South University, Changsha, 410011, China; Institute of Vascular Diseases, Central South University, Changsha, 410011, ChinaDepartment of Vascular Surgery, The Second Xiangya Hospital, Central South University, Changsha, 410011, China; Institute of Vascular Diseases, Central South University, Changsha, 410011, ChinaDepartment of Vascular Surgery, The Second Xiangya Hospital, Central South University, Changsha, 410011, China; Institute of Vascular Diseases, Central South University, Changsha, 410011, ChinaDepartment of Vascular Surgery, The Second Xiangya Hospital, Central South University, Changsha, 410011, China; Institute of Vascular Diseases, Central South University, Changsha, 410011, ChinaDepartment of Vascular Surgery, The Second Xiangya Hospital, Central South University, Changsha, 410011, China; Institute of Vascular Diseases, Central South University, Changsha, 410011, China; Center of Vascular Surgery, Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100037, China; Corresponding author. Department of Vascular Surgery, The Second Xiangya Hospital, Central South University, Changsha 410011, China. Institute of Vascular Diseases, Central South University, Changsha 410011, China. Center of Vascular Surgery, Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China.Decellularized tissue-engineered vascular grafts (dTEVGs) exhibit superior biocompatibility, anti-infection properties and repair potential, contributing to better patency and making them a more ideal choice for arteriovenous grafts (AVGs) in hemodialysis compared to chemically synthesized grafts. However, the unsatisfactory reendothelialization and smooth muscle remodeling of current dTEVGs limit their advantages. In this study, we investigated the use of elastase to improve the porosity of elastic fiber layers in dTEVGs, aiming to promote cell infiltration and achieve superior reendothelialization and smooth muscle remodeling. Our findings revealed that elastase treatment induced scattered cracks and holes in the elastic fiber layers of dTEVGs. Porous dTEVGs demonstrated increased cell infiltration in rat subcutaneous tissue. In the rat AVG models, mildly elastase-treated dTEVGs significantly improved cell infiltration and graft remodeling, including adequate smooth muscle cell (SMC) repopulation, impressive reendothelization and regeneration of the extracellular matrix, without stenosis, dilation or disintegration of the grafts. This study demonstrates that porous dTEVGs promote reendothelization, smooth muscle remodeling and extracellular matrix regeneration while retaining a stable graft structure, enhancing durability and puncture resistance in hemodialysis.http://www.sciencedirect.com/science/article/pii/S2590006424004630Tissue-engineered vascular graftReendothelializationRemodelingArteriovenous graftHemodialysis
spellingShingle Tun Wang
Sheng Liao
Peng Lu
Zhenyu He
Siyuan Cheng
Tianjian Wang
Zibo Cheng
Yangyang An
Mo Wang
Chang Shu
Improved porosity promotes reendothelialization and smooth muscle remodeling in decellularized tissue-engineered vascular grafts
Materials Today Bio
Tissue-engineered vascular graft
Reendothelialization
Remodeling
Arteriovenous graft
Hemodialysis
title Improved porosity promotes reendothelialization and smooth muscle remodeling in decellularized tissue-engineered vascular grafts
title_full Improved porosity promotes reendothelialization and smooth muscle remodeling in decellularized tissue-engineered vascular grafts
title_fullStr Improved porosity promotes reendothelialization and smooth muscle remodeling in decellularized tissue-engineered vascular grafts
title_full_unstemmed Improved porosity promotes reendothelialization and smooth muscle remodeling in decellularized tissue-engineered vascular grafts
title_short Improved porosity promotes reendothelialization and smooth muscle remodeling in decellularized tissue-engineered vascular grafts
title_sort improved porosity promotes reendothelialization and smooth muscle remodeling in decellularized tissue engineered vascular grafts
topic Tissue-engineered vascular graft
Reendothelialization
Remodeling
Arteriovenous graft
Hemodialysis
url http://www.sciencedirect.com/science/article/pii/S2590006424004630
work_keys_str_mv AT tunwang improvedporositypromotesreendothelializationandsmoothmuscleremodelingindecellularizedtissueengineeredvasculargrafts
AT shengliao improvedporositypromotesreendothelializationandsmoothmuscleremodelingindecellularizedtissueengineeredvasculargrafts
AT penglu improvedporositypromotesreendothelializationandsmoothmuscleremodelingindecellularizedtissueengineeredvasculargrafts
AT zhenyuhe improvedporositypromotesreendothelializationandsmoothmuscleremodelingindecellularizedtissueengineeredvasculargrafts
AT siyuancheng improvedporositypromotesreendothelializationandsmoothmuscleremodelingindecellularizedtissueengineeredvasculargrafts
AT tianjianwang improvedporositypromotesreendothelializationandsmoothmuscleremodelingindecellularizedtissueengineeredvasculargrafts
AT zibocheng improvedporositypromotesreendothelializationandsmoothmuscleremodelingindecellularizedtissueengineeredvasculargrafts
AT yangyangan improvedporositypromotesreendothelializationandsmoothmuscleremodelingindecellularizedtissueengineeredvasculargrafts
AT mowang improvedporositypromotesreendothelializationandsmoothmuscleremodelingindecellularizedtissueengineeredvasculargrafts
AT changshu improvedporositypromotesreendothelializationandsmoothmuscleremodelingindecellularizedtissueengineeredvasculargrafts