<i>Aspergillus oryzae</i> Fermented <i>Plumula Nelumbinis</i> Against Atopic Dermatitis Through AKT/mTOR and Jun Pathways
<b>Background/Objectives:</b> Atopic dermatitis (AD) is a chronic inflammatory skin disorder that has attracted global attention, and alkaloids from <i>Plumula Nelumbinis</i> have been shown to have anti-inflammatory activity. Fermentation has been used for the structural mod...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Pharmaceuticals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8247/18/1/20 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832587741188063232 |
|---|---|
| author | Fengfeng Chen Jing Liu Xinwei Yu Honglei Jia Cheng Yang Bingtian Zhao |
| author_facet | Fengfeng Chen Jing Liu Xinwei Yu Honglei Jia Cheng Yang Bingtian Zhao |
| author_sort | Fengfeng Chen |
| collection | DOAJ |
| description | <b>Background/Objectives:</b> Atopic dermatitis (AD) is a chronic inflammatory skin disorder that has attracted global attention, and alkaloids from <i>Plumula Nelumbinis</i> have been shown to have anti-inflammatory activity. Fermentation has been used for the structural modification of natural compounds to improve bioavailability and activity, but the AD therapeutic efficacy and mechanism of the fermented <i>Plumula Nelumbinis</i> (FPN) are still unclear. <b>Methods:</b> The potential targets of FPN for AD were preliminarily screened using network pharmacology, and then PCR and WB were used to prove the therapeutic effect of FPN in AD. <b>Results:</b> Network pharmacology indicated that mTOR and Jun were key targets for AD. The experiments in vitro showed that FPN could effectively block AKT/mTOR and AKT/Jun-mediated inflammatory signaling pathways. Moreover, FPN can also alleviate SDS-induced inflammation in zebrafish. It is also found that the anti-inflammatory activity of <i>Plumula Nelumbinis</i> was enhanced by <i>Aspergillus oryzae</i> fermentation, and the oil phase of the fermentation product showed better activity, which may be due to microbial fermentation changing the structure of the original alkaloids. <b>Conclusions:</b> This study elucidated the potential mechanisms of alkaloids derived from fermented <i>Plumula Nelumbinis</i> against AD; it may also provide a scientific basis for the development of new drugs for AD. |
| format | Article |
| id | doaj-art-d00921e13a6c4f0fb87298ff97276200 |
| institution | Kabale University |
| issn | 1424-8247 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj-art-d00921e13a6c4f0fb87298ff972762002025-01-24T13:45:02ZengMDPI AGPharmaceuticals1424-82472024-12-011812010.3390/ph18010020<i>Aspergillus oryzae</i> Fermented <i>Plumula Nelumbinis</i> Against Atopic Dermatitis Through AKT/mTOR and Jun PathwaysFengfeng Chen0Jing Liu1Xinwei Yu2Honglei Jia3Cheng Yang4Bingtian Zhao5Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, ChinaKey Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, ChinaKey Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, ChinaShanghai Fulai BioHighTech Co., Ltd., Shanghai 201400, ChinaKey Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, ChinaKey Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, China<b>Background/Objectives:</b> Atopic dermatitis (AD) is a chronic inflammatory skin disorder that has attracted global attention, and alkaloids from <i>Plumula Nelumbinis</i> have been shown to have anti-inflammatory activity. Fermentation has been used for the structural modification of natural compounds to improve bioavailability and activity, but the AD therapeutic efficacy and mechanism of the fermented <i>Plumula Nelumbinis</i> (FPN) are still unclear. <b>Methods:</b> The potential targets of FPN for AD were preliminarily screened using network pharmacology, and then PCR and WB were used to prove the therapeutic effect of FPN in AD. <b>Results:</b> Network pharmacology indicated that mTOR and Jun were key targets for AD. The experiments in vitro showed that FPN could effectively block AKT/mTOR and AKT/Jun-mediated inflammatory signaling pathways. Moreover, FPN can also alleviate SDS-induced inflammation in zebrafish. It is also found that the anti-inflammatory activity of <i>Plumula Nelumbinis</i> was enhanced by <i>Aspergillus oryzae</i> fermentation, and the oil phase of the fermentation product showed better activity, which may be due to microbial fermentation changing the structure of the original alkaloids. <b>Conclusions:</b> This study elucidated the potential mechanisms of alkaloids derived from fermented <i>Plumula Nelumbinis</i> against AD; it may also provide a scientific basis for the development of new drugs for AD.https://www.mdpi.com/1424-8247/18/1/20<i>Plumula Nelumbinis</i>alkaloidfermentationatopic dermatitisnetwork pharmacologymechanism |
| spellingShingle | Fengfeng Chen Jing Liu Xinwei Yu Honglei Jia Cheng Yang Bingtian Zhao <i>Aspergillus oryzae</i> Fermented <i>Plumula Nelumbinis</i> Against Atopic Dermatitis Through AKT/mTOR and Jun Pathways Pharmaceuticals <i>Plumula Nelumbinis</i> alkaloid fermentation atopic dermatitis network pharmacology mechanism |
| title | <i>Aspergillus oryzae</i> Fermented <i>Plumula Nelumbinis</i> Against Atopic Dermatitis Through AKT/mTOR and Jun Pathways |
| title_full | <i>Aspergillus oryzae</i> Fermented <i>Plumula Nelumbinis</i> Against Atopic Dermatitis Through AKT/mTOR and Jun Pathways |
| title_fullStr | <i>Aspergillus oryzae</i> Fermented <i>Plumula Nelumbinis</i> Against Atopic Dermatitis Through AKT/mTOR and Jun Pathways |
| title_full_unstemmed | <i>Aspergillus oryzae</i> Fermented <i>Plumula Nelumbinis</i> Against Atopic Dermatitis Through AKT/mTOR and Jun Pathways |
| title_short | <i>Aspergillus oryzae</i> Fermented <i>Plumula Nelumbinis</i> Against Atopic Dermatitis Through AKT/mTOR and Jun Pathways |
| title_sort | i aspergillus oryzae i fermented i plumula nelumbinis i against atopic dermatitis through akt mtor and jun pathways |
| topic | <i>Plumula Nelumbinis</i> alkaloid fermentation atopic dermatitis network pharmacology mechanism |
| url | https://www.mdpi.com/1424-8247/18/1/20 |
| work_keys_str_mv | AT fengfengchen iaspergillusoryzaeifermentediplumulanelumbinisiagainstatopicdermatitisthroughaktmtorandjunpathways AT jingliu iaspergillusoryzaeifermentediplumulanelumbinisiagainstatopicdermatitisthroughaktmtorandjunpathways AT xinweiyu iaspergillusoryzaeifermentediplumulanelumbinisiagainstatopicdermatitisthroughaktmtorandjunpathways AT hongleijia iaspergillusoryzaeifermentediplumulanelumbinisiagainstatopicdermatitisthroughaktmtorandjunpathways AT chengyang iaspergillusoryzaeifermentediplumulanelumbinisiagainstatopicdermatitisthroughaktmtorandjunpathways AT bingtianzhao iaspergillusoryzaeifermentediplumulanelumbinisiagainstatopicdermatitisthroughaktmtorandjunpathways |