Transient proteolysis reduction of Nicotiana benthamiana-produced CAP256 broadly neutralizing antibodies using CRISPR/Cas9

Transient proteolysis reduction of Nicotiana benthamiana-produced CAP256 broadly neutralizing antibodies using CRISPR/Cas9

The hypersensitive response is elicited by Agrobacterium infiltration of Nicotiana benthamiana, including the induction and accumulation of pathogenesis-related proteins, such as proteases. This includes the induction of the expression of several cysteine proteases from the C1 (papain-like cysteine...

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Bibliographic Details
Main Authors: Advaita Acarya Singh, Priyen Pillay, Previn Naicker, Kabamba Alexandre, Kanyane Malatji, Lukas Mach, Herta Steinkellner, Juan Vorster, Rachel Chikwamba, Tsepo L. Tsekoa
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-08-01
Series:Frontiers in Plant Science
Subjects:
plant biotechnology
CRISPR/Cas9
genome editing
Nicotiana benthamiana
proteases
immunoglobulin G
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2022.953654/full
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https://www.frontiersin.org/articles/10.3389/fpls.2022.953654/full

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