Biases from Oxford Nanopore library preparation kits and their effects on microbiome and genome analysis

Biases from Oxford Nanopore library preparation kits and their effects on microbiome and genome analysis

Abstract Background Oxford Nanopore sequencing is a long-read sequencing technology that does not rely on a polymerase to generate sequence data. Sequencing library preparation methods used in Oxford Nanopore sequencing rely on the addition of a motor protein bound to an adapter sequence, which is a...

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Bibliographic Details
Main Authors: Ziming Chen, Chian Teng Ong, Loan To Nguyen, Harrison J. Lamb, O. González-Recio, M. Gutiérrez-Rivas, Sarah J. Meale, Elizabeth M. Ross
Format: Article
Language:English
Published: BMC 2025-05-01
Series:BMC Genomics
Subjects:
Oxford Nanopore sequencing
DNA extraction
Library preparation
GC bias
Microbiome
Online Access:https://doi.org/10.1186/s12864-025-11649-z
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https://doi.org/10.1186/s12864-025-11649-z

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