Rapid, Efficient, and Universally Applicable Genetic Engineering of Intestinal Organoid with a Sequential Monolayer to Three-Dimensional Strategy

Genetically modified intestinal organoids are being explored as potential surrogates of immortalized cell lines and gene-engineered animals. However, genetic manipulation of intestinal organoids is time-consuming, and the efficiency is far beyond satisfactory. To ensure the yield of the genetically...

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Bibliographic Details
Main Authors: Weili Han, Guofang Lu, Sheng Zhao, Rui Wang, Haohao Zhang, Kun Liu, Yongzhan Nie, Jiaqiang Dong
Format: Article
Language:English
Published: Wiley 2024-01-01
Series:Stem Cells International
Online Access:http://dx.doi.org/10.1155/2024/2005845
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