Innate killing of Leishmania donovani by macrophages of the splenic marginal zone requires IRF-7.
Highly phagocytic macrophages line the marginal zone (MZ) of the spleen and the lymph node subcapsular sinus. Although these macrophages have been attributed with a variety of functions, including the uptake and clearance of blood and lymph-borne pathogens, little is known about the effector mechani...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2010-03-01
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| Series: | PLoS Pathogens |
| Online Access: | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1000813&type=printable |
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| author | Rebecca Phillips Mattias Svensson Naveed Aziz Asher Maroof Najmeeyah Brown Lynette Beattie Nathalie Signoret Paul M Kaye |
| author_facet | Rebecca Phillips Mattias Svensson Naveed Aziz Asher Maroof Najmeeyah Brown Lynette Beattie Nathalie Signoret Paul M Kaye |
| author_sort | Rebecca Phillips |
| collection | DOAJ |
| description | Highly phagocytic macrophages line the marginal zone (MZ) of the spleen and the lymph node subcapsular sinus. Although these macrophages have been attributed with a variety of functions, including the uptake and clearance of blood and lymph-borne pathogens, little is known about the effector mechanisms they employ after pathogen uptake. Here, we have combined gene expression profiling and RNAi using a stromal macrophage cell line with in situ analysis of the leishmanicidal activity of marginal zone macrophages (MZM) and marginal metallophilic macrophages (MMM) in wild type and gene targeted mice. Our data demonstrate a critical role for interferon regulatory factor-7 (IRF-7) in regulating the killing of intracellular Leishmania donovani by these specialised splenic macrophage sub-populations. This study, therefore, identifies a new role for IRF-7 as a regulator of innate microbicidal activity against this, and perhaps other, non-viral intracellular pathogens. This study also highlights the importance of selecting appropriate macrophage populations when studying pathogen interactions with this functionally diverse lineage of cells. |
| format | Article |
| id | doaj-art-ceb3a3cc3b474c08854e66698d014a90 |
| institution | DOAJ |
| issn | 1553-7366 1553-7374 |
| language | English |
| publishDate | 2010-03-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj-art-ceb3a3cc3b474c08854e66698d014a902025-08-20T03:19:52ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742010-03-0163e100081310.1371/journal.ppat.1000813Innate killing of Leishmania donovani by macrophages of the splenic marginal zone requires IRF-7.Rebecca PhillipsMattias SvenssonNaveed AzizAsher MaroofNajmeeyah BrownLynette BeattieNathalie SignoretPaul M KayeHighly phagocytic macrophages line the marginal zone (MZ) of the spleen and the lymph node subcapsular sinus. Although these macrophages have been attributed with a variety of functions, including the uptake and clearance of blood and lymph-borne pathogens, little is known about the effector mechanisms they employ after pathogen uptake. Here, we have combined gene expression profiling and RNAi using a stromal macrophage cell line with in situ analysis of the leishmanicidal activity of marginal zone macrophages (MZM) and marginal metallophilic macrophages (MMM) in wild type and gene targeted mice. Our data demonstrate a critical role for interferon regulatory factor-7 (IRF-7) in regulating the killing of intracellular Leishmania donovani by these specialised splenic macrophage sub-populations. This study, therefore, identifies a new role for IRF-7 as a regulator of innate microbicidal activity against this, and perhaps other, non-viral intracellular pathogens. This study also highlights the importance of selecting appropriate macrophage populations when studying pathogen interactions with this functionally diverse lineage of cells.https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1000813&type=printable |
| spellingShingle | Rebecca Phillips Mattias Svensson Naveed Aziz Asher Maroof Najmeeyah Brown Lynette Beattie Nathalie Signoret Paul M Kaye Innate killing of Leishmania donovani by macrophages of the splenic marginal zone requires IRF-7. PLoS Pathogens |
| title | Innate killing of Leishmania donovani by macrophages of the splenic marginal zone requires IRF-7. |
| title_full | Innate killing of Leishmania donovani by macrophages of the splenic marginal zone requires IRF-7. |
| title_fullStr | Innate killing of Leishmania donovani by macrophages of the splenic marginal zone requires IRF-7. |
| title_full_unstemmed | Innate killing of Leishmania donovani by macrophages of the splenic marginal zone requires IRF-7. |
| title_short | Innate killing of Leishmania donovani by macrophages of the splenic marginal zone requires IRF-7. |
| title_sort | innate killing of leishmania donovani by macrophages of the splenic marginal zone requires irf 7 |
| url | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1000813&type=printable |
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