Assessment of the Probiotic Properties of Pediococcus acidilactici, Pediococcus pentosaceus, and Lactiplantibacillus plantarum Strains Isolated From Fermented Maize Grains
This study aimed to evaluate the probiotic properties of 11 lactic acid bacteria (LAB) strains isolated from corn kernels. After phenotypic and biochemical characterization confirmed by 16S rRNA gene sequencing, the results revealed that eight isolates (AA1, AA4, AA5, AA7, AA8, BB1, Z3, and Z4) belo...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-05-01
|
| Series: | Journal of Food Protection |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0362028X25000663 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849387340750913536 |
|---|---|
| author | Amidou S. Ouili Iliassou Mogmenga Assiètta Ouattara Cheik Omar Tidiane Compaoré Ynoussa Maiga Mahamadi Nikiema Aboubakar Sidiki Ouattara |
| author_facet | Amidou S. Ouili Iliassou Mogmenga Assiètta Ouattara Cheik Omar Tidiane Compaoré Ynoussa Maiga Mahamadi Nikiema Aboubakar Sidiki Ouattara |
| author_sort | Amidou S. Ouili |
| collection | DOAJ |
| description | This study aimed to evaluate the probiotic properties of 11 lactic acid bacteria (LAB) strains isolated from corn kernels. After phenotypic and biochemical characterization confirmed by 16S rRNA gene sequencing, the results revealed that eight isolates (AA1, AA4, AA5, AA7, AA8, BB1, Z3, and Z4) belonged to Pediococcus acidilactici, two (Z2 and Z5) to Pediococcus pentosaceus, and one (AA6) to Lactiplantibacillus plantarum. Antibiotic sensitivity analysis showed general resistance to ciprofloxacin, gentamicin, and colistin, but strains such as AA4, AA6, Z2, and AA1 were sensitive to ampicillin, amoxicillin/clavulanic acid, and ceftriaxone. No signs of hemolytic activity were observed, confirming the safety of the strains. Simulated gastrointestinal tolerance tests demonstrated high survival rates: between 55.64% and 96.61% under 0.3% pepsin at pH 2.5, between 91.24% and 96.67% with bile salts (0.3%), and between 72.95% and 99.66% with phenol (0.4%). Autoaggregation capacities ranged from 54.87% to 90.57%, and coaggregation rates with E. coli and S. enterica were also significant. The strains exhibited hydrophobicity rates between 46.65% and 77.17%, notable antioxidant capacities (58.46–69.6%), and inhibited the growth of foodborne pathogens. Finally, enzymatic profiles revealed proteolytic and lipolytic activities. The isolates Z2 and Z3 stand out due to their wide range of probiotic characteristics, making them promising candidates for future research. |
| format | Article |
| id | doaj-art-ce90fe06849d46d5b6b1e877d1708799 |
| institution | Kabale University |
| issn | 0362-028X |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Food Protection |
| spelling | doaj-art-ce90fe06849d46d5b6b1e877d17087992025-08-20T03:53:51ZengElsevierJournal of Food Protection0362-028X2025-05-0188610051410.1016/j.jfp.2025.100514Assessment of the Probiotic Properties of Pediococcus acidilactici, Pediococcus pentosaceus, and Lactiplantibacillus plantarum Strains Isolated From Fermented Maize GrainsAmidou S. Ouili0Iliassou Mogmenga1Assiètta Ouattara2Cheik Omar Tidiane Compaoré3Ynoussa Maiga4Mahamadi Nikiema5Aboubakar Sidiki Ouattara6Laboratoire de Microbiologie et de Biotechnologies Microbiennes, Université Joseph KI-ZERBO, 03 BP 7021, Ouagadougou 03, Burkina Faso; Corresponding author.Laboratoire de Microbiologie et de Biotechnologies Microbiennes, Université Joseph KI-ZERBO, 03 BP 7021, Ouagadougou 03, Burkina Faso; Centre Universitaire de Banfora, Université Nazi BONI, Bobo-Dioulasso 01 BP 1091, Burkina FasoLaboratoire de Microbiologie et de Biotechnologies Microbiennes, Université Joseph KI-ZERBO, 03 BP 7021, Ouagadougou 03, Burkina FasoLaboratoire de Microbiologie et de Biotechnologies Microbiennes, Université Joseph KI-ZERBO, 03 BP 7021, Ouagadougou 03, Burkina FasoLaboratoire de Microbiologie et de Biotechnologies Microbiennes, Université Joseph KI-ZERBO, 03 BP 7021, Ouagadougou 03, Burkina FasoLaboratoire de Microbiologie et de Biotechnologies Microbiennes, Université Joseph KI-ZERBO, 03 BP 7021, Ouagadougou 03, Burkina Faso; Institut Supérieur de Développement Durable (ISDD), Université Yembila Abdoulaye Toguyeni, BP 54 Fada N’Gourma, Burkina FasoLaboratoire de Microbiologie et de Biotechnologies Microbiennes, Université Joseph KI-ZERBO, 03 BP 7021, Ouagadougou 03, Burkina FasoThis study aimed to evaluate the probiotic properties of 11 lactic acid bacteria (LAB) strains isolated from corn kernels. After phenotypic and biochemical characterization confirmed by 16S rRNA gene sequencing, the results revealed that eight isolates (AA1, AA4, AA5, AA7, AA8, BB1, Z3, and Z4) belonged to Pediococcus acidilactici, two (Z2 and Z5) to Pediococcus pentosaceus, and one (AA6) to Lactiplantibacillus plantarum. Antibiotic sensitivity analysis showed general resistance to ciprofloxacin, gentamicin, and colistin, but strains such as AA4, AA6, Z2, and AA1 were sensitive to ampicillin, amoxicillin/clavulanic acid, and ceftriaxone. No signs of hemolytic activity were observed, confirming the safety of the strains. Simulated gastrointestinal tolerance tests demonstrated high survival rates: between 55.64% and 96.61% under 0.3% pepsin at pH 2.5, between 91.24% and 96.67% with bile salts (0.3%), and between 72.95% and 99.66% with phenol (0.4%). Autoaggregation capacities ranged from 54.87% to 90.57%, and coaggregation rates with E. coli and S. enterica were also significant. The strains exhibited hydrophobicity rates between 46.65% and 77.17%, notable antioxidant capacities (58.46–69.6%), and inhibited the growth of foodborne pathogens. Finally, enzymatic profiles revealed proteolytic and lipolytic activities. The isolates Z2 and Z3 stand out due to their wide range of probiotic characteristics, making them promising candidates for future research.http://www.sciencedirect.com/science/article/pii/S0362028X25000663AntimicrobialBurkina FasoMaizeMicroorganismsProbiotics |
| spellingShingle | Amidou S. Ouili Iliassou Mogmenga Assiètta Ouattara Cheik Omar Tidiane Compaoré Ynoussa Maiga Mahamadi Nikiema Aboubakar Sidiki Ouattara Assessment of the Probiotic Properties of Pediococcus acidilactici, Pediococcus pentosaceus, and Lactiplantibacillus plantarum Strains Isolated From Fermented Maize Grains Journal of Food Protection Antimicrobial Burkina Faso Maize Microorganisms Probiotics |
| title | Assessment of the Probiotic Properties of Pediococcus acidilactici, Pediococcus pentosaceus, and Lactiplantibacillus plantarum Strains Isolated From Fermented Maize Grains |
| title_full | Assessment of the Probiotic Properties of Pediococcus acidilactici, Pediococcus pentosaceus, and Lactiplantibacillus plantarum Strains Isolated From Fermented Maize Grains |
| title_fullStr | Assessment of the Probiotic Properties of Pediococcus acidilactici, Pediococcus pentosaceus, and Lactiplantibacillus plantarum Strains Isolated From Fermented Maize Grains |
| title_full_unstemmed | Assessment of the Probiotic Properties of Pediococcus acidilactici, Pediococcus pentosaceus, and Lactiplantibacillus plantarum Strains Isolated From Fermented Maize Grains |
| title_short | Assessment of the Probiotic Properties of Pediococcus acidilactici, Pediococcus pentosaceus, and Lactiplantibacillus plantarum Strains Isolated From Fermented Maize Grains |
| title_sort | assessment of the probiotic properties of pediococcus acidilactici pediococcus pentosaceus and lactiplantibacillus plantarum strains isolated from fermented maize grains |
| topic | Antimicrobial Burkina Faso Maize Microorganisms Probiotics |
| url | http://www.sciencedirect.com/science/article/pii/S0362028X25000663 |
| work_keys_str_mv | AT amidousouili assessmentoftheprobioticpropertiesofpediococcusacidilacticipediococcuspentosaceusandlactiplantibacillusplantarumstrainsisolatedfromfermentedmaizegrains AT iliassoumogmenga assessmentoftheprobioticpropertiesofpediococcusacidilacticipediococcuspentosaceusandlactiplantibacillusplantarumstrainsisolatedfromfermentedmaizegrains AT assiettaouattara assessmentoftheprobioticpropertiesofpediococcusacidilacticipediococcuspentosaceusandlactiplantibacillusplantarumstrainsisolatedfromfermentedmaizegrains AT cheikomartidianecompaore assessmentoftheprobioticpropertiesofpediococcusacidilacticipediococcuspentosaceusandlactiplantibacillusplantarumstrainsisolatedfromfermentedmaizegrains AT ynoussamaiga assessmentoftheprobioticpropertiesofpediococcusacidilacticipediococcuspentosaceusandlactiplantibacillusplantarumstrainsisolatedfromfermentedmaizegrains AT mahamadinikiema assessmentoftheprobioticpropertiesofpediococcusacidilacticipediococcuspentosaceusandlactiplantibacillusplantarumstrainsisolatedfromfermentedmaizegrains AT aboubakarsidikiouattara assessmentoftheprobioticpropertiesofpediococcusacidilacticipediococcuspentosaceusandlactiplantibacillusplantarumstrainsisolatedfromfermentedmaizegrains |