Single-cell RNA sequencing of intramedullary canal tissue to improve methods for studying fracture repair biology
The ability to study the bone microenvironment of failed fracture healing may lead to biomarkers for fracture nonunion. Herein the authors describe a technique for isolating individual cells suitable for single-cell RNA sequencing analyses from intramedullary canal tissue collected by reaming during...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-08-01
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| Series: | BioTechniques |
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| Online Access: | https://www.future-science.com/doi/10.2144/btn-2021-0002 |
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| _version_ | 1850152832962920448 |
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| author | James M Dominguez Sharon M Moe Neal X Chen Todd O McKinley Krista M Brown Yunlong Liu Hongyu Gao Roman M Natoli |
| author_facet | James M Dominguez Sharon M Moe Neal X Chen Todd O McKinley Krista M Brown Yunlong Liu Hongyu Gao Roman M Natoli |
| author_sort | James M Dominguez |
| collection | DOAJ |
| description | The ability to study the bone microenvironment of failed fracture healing may lead to biomarkers for fracture nonunion. Herein the authors describe a technique for isolating individual cells suitable for single-cell RNA sequencing analyses from intramedullary canal tissue collected by reaming during surgery. The purpose was to detail challenges and solutions inherent to the collection and processing of intramedullary canal tissue samples. The authors then examined single-cell RNA sequencing data from fresh and reanimated samples to demonstrate the feasibility of this approach for prospective studies. |
| format | Article |
| id | doaj-art-cdd5b915484a40638d1700d57d37cbf7 |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2021-08-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-cdd5b915484a40638d1700d57d37cbf72025-08-20T02:25:51ZengTaylor & Francis GroupBioTechniques0736-62051940-98182021-08-0171243143810.2144/btn-2021-0002Single-cell RNA sequencing of intramedullary canal tissue to improve methods for studying fracture repair biologyJames M Dominguez0Sharon M Moe1Neal X Chen2Todd O McKinley3Krista M Brown4Yunlong Liu5Hongyu Gao6Roman M Natoli71Department of Medicine, Division of Nephrology, Indiana University School of Medicine, Indianapolis, IN 46202, USA1Department of Medicine, Division of Nephrology, Indiana University School of Medicine, Indianapolis, IN 46202, USA1Department of Medicine, Division of Nephrology, Indiana University School of Medicine, Indianapolis, IN 46202, USA2Department of Orthopaedic Surgery, Indiana University Health Methodist Hospital, Indianapolis, IN 46202, USA2Department of Orthopaedic Surgery, Indiana University Health Methodist Hospital, Indianapolis, IN 46202, USA3Department of Medical & Molecular Genetics, Center for Medical Genomics, Indiana University School of Medicine, Indianapolis, IN 46202, USA3Department of Medical & Molecular Genetics, Center for Medical Genomics, Indiana University School of Medicine, Indianapolis, IN 46202, USA2Department of Orthopaedic Surgery, Indiana University Health Methodist Hospital, Indianapolis, IN 46202, USAThe ability to study the bone microenvironment of failed fracture healing may lead to biomarkers for fracture nonunion. Herein the authors describe a technique for isolating individual cells suitable for single-cell RNA sequencing analyses from intramedullary canal tissue collected by reaming during surgery. The purpose was to detail challenges and solutions inherent to the collection and processing of intramedullary canal tissue samples. The authors then examined single-cell RNA sequencing data from fresh and reanimated samples to demonstrate the feasibility of this approach for prospective studies.https://www.future-science.com/doi/10.2144/btn-2021-0002cell isolationcryopreservationfracturenonunionsingle-cell RNA sequencing |
| spellingShingle | James M Dominguez Sharon M Moe Neal X Chen Todd O McKinley Krista M Brown Yunlong Liu Hongyu Gao Roman M Natoli Single-cell RNA sequencing of intramedullary canal tissue to improve methods for studying fracture repair biology BioTechniques cell isolation cryopreservation fracture nonunion single-cell RNA sequencing |
| title | Single-cell RNA sequencing of intramedullary canal tissue to improve methods for studying fracture repair biology |
| title_full | Single-cell RNA sequencing of intramedullary canal tissue to improve methods for studying fracture repair biology |
| title_fullStr | Single-cell RNA sequencing of intramedullary canal tissue to improve methods for studying fracture repair biology |
| title_full_unstemmed | Single-cell RNA sequencing of intramedullary canal tissue to improve methods for studying fracture repair biology |
| title_short | Single-cell RNA sequencing of intramedullary canal tissue to improve methods for studying fracture repair biology |
| title_sort | single cell rna sequencing of intramedullary canal tissue to improve methods for studying fracture repair biology |
| topic | cell isolation cryopreservation fracture nonunion single-cell RNA sequencing |
| url | https://www.future-science.com/doi/10.2144/btn-2021-0002 |
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