Single-cell RNA sequencing of intramedullary canal tissue to improve methods for studying fracture repair biology

Single-cell RNA sequencing of intramedullary canal tissue to improve methods for studying fracture repair biology

The ability to study the bone microenvironment of failed fracture healing may lead to biomarkers for fracture nonunion. Herein the authors describe a technique for isolating individual cells suitable for single-cell RNA sequencing analyses from intramedullary canal tissue collected by reaming during...

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Bibliographic Details
Main Authors: James M Dominguez, Sharon M Moe, Neal X Chen, Todd O McKinley, Krista M Brown, Yunlong Liu, Hongyu Gao, Roman M Natoli
Format: Article
Language:English
Published: Taylor & Francis Group 2021-08-01
Series:BioTechniques
Subjects:
cell isolation
cryopreservation
fracture
nonunion
single-cell RNA sequencing
Online Access:https://www.future-science.com/doi/10.2144/btn-2021-0002
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https://www.future-science.com/doi/10.2144/btn-2021-0002

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