Encapsulated LyeTx III Peptide: Cytotoxic Agent Isolated from <i>Lycosa erythrognatha</i> Spider Venom
The discovery of novel cytotoxic drugs is of paramount importance in contemporary medical research, particularly in the search for treatments with fewer side effects and higher specificity. Antimicrobial peptides are an interesting class of molecules for this endeavor. In this context, the LyeTx III...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Toxins |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6651/17/1/32 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832587457943568384 |
|---|---|
| author | Daniel Moreira dos Santos Livia Ramos Santiago Nayara Araújo dos Santos Wanderson Romão Jarbas Magalhães Resende Maria Elena de Lima Márcia Helena Borges Rosy Iara Maciel de Azambuja Ribeiro |
| author_facet | Daniel Moreira dos Santos Livia Ramos Santiago Nayara Araújo dos Santos Wanderson Romão Jarbas Magalhães Resende Maria Elena de Lima Márcia Helena Borges Rosy Iara Maciel de Azambuja Ribeiro |
| author_sort | Daniel Moreira dos Santos |
| collection | DOAJ |
| description | The discovery of novel cytotoxic drugs is of paramount importance in contemporary medical research, particularly in the search for treatments with fewer side effects and higher specificity. Antimicrobial peptides are an interesting class of molecules for this endeavor. In this context, the LyeTx III, a new peptide extracted from the venom of the <i>Lycosa erythrognatha</i> spider, stands out. The peptide exhibits typical antimicrobial traits: a positive net charge and amphipathic α -helix structure in lipid-like environments. Its unique sequence (GKAMKAIAKFLGR-NH<sub>2</sub>), identified via mass spectrometry and Edman degradation, shows limited similarity to existing peptides. Significantly, when liposome-encapsulated, LyeTx III demonstrates selective activity against tumor cells in culture. Our MTT results showed that the cytotoxicity of the peptide increased against HN13 cells when administered as liposomes, with their viability in HN13 cells alone being 98%, compared to 38% in liposome-encapsulated form. This finding underscores that the LyeTx III peptide may be a good candidate for the development of new drugs against cancer. Its activity when encapsulated is promising, as it can increase its half-life in the body and can also be targeted to specific tumors. |
| format | Article |
| id | doaj-art-cdab7abb27ab489a8205a4e71efe7e2f |
| institution | Kabale University |
| issn | 2072-6651 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj-art-cdab7abb27ab489a8205a4e71efe7e2f2025-01-24T13:51:16ZengMDPI AGToxins2072-66512025-01-011713210.3390/toxins17010032Encapsulated LyeTx III Peptide: Cytotoxic Agent Isolated from <i>Lycosa erythrognatha</i> Spider VenomDaniel Moreira dos Santos0Livia Ramos Santiago1Nayara Araújo dos Santos2Wanderson Romão3Jarbas Magalhães Resende4Maria Elena de Lima5Márcia Helena Borges6Rosy Iara Maciel de Azambuja Ribeiro7Department of Experimental Pathology, Federal University of São João del-Rei, Divinópolis 36301-158, BrazilDepartment of Experimental Pathology, Federal University of São João del-Rei, Divinópolis 36301-158, BrazilPetroleomics and Forensics Laboratory, Federal University of Espírito Santo, Vitória 29075-910, BrazilPetroleomics and Forensics Laboratory, Federal University of Espírito Santo, Vitória 29075-910, BrazilDepartment of Chemistry, Federal University of Minas Gerais (UFMG), Belo Horizonte 30110-005, BrazilPrograma de Pós-Graduação em Medicina-Biomedicina, Faculdade Santa Casa de Belo Horizonte, Belo Horizonte 30110-005, BrazilProteomics and Arachnid Laboratory, Ezequiel Dias Foundation, Belo Horizonte 30110-005, BrazilDepartment of Experimental Pathology, Federal University of São João del-Rei, Divinópolis 36301-158, BrazilThe discovery of novel cytotoxic drugs is of paramount importance in contemporary medical research, particularly in the search for treatments with fewer side effects and higher specificity. Antimicrobial peptides are an interesting class of molecules for this endeavor. In this context, the LyeTx III, a new peptide extracted from the venom of the <i>Lycosa erythrognatha</i> spider, stands out. The peptide exhibits typical antimicrobial traits: a positive net charge and amphipathic α -helix structure in lipid-like environments. Its unique sequence (GKAMKAIAKFLGR-NH<sub>2</sub>), identified via mass spectrometry and Edman degradation, shows limited similarity to existing peptides. Significantly, when liposome-encapsulated, LyeTx III demonstrates selective activity against tumor cells in culture. Our MTT results showed that the cytotoxicity of the peptide increased against HN13 cells when administered as liposomes, with their viability in HN13 cells alone being 98%, compared to 38% in liposome-encapsulated form. This finding underscores that the LyeTx III peptide may be a good candidate for the development of new drugs against cancer. Its activity when encapsulated is promising, as it can increase its half-life in the body and can also be targeted to specific tumors.https://www.mdpi.com/2072-6651/17/1/32antimicrobial peptidesantitumor activityspider venomencapsulated peptide |
| spellingShingle | Daniel Moreira dos Santos Livia Ramos Santiago Nayara Araújo dos Santos Wanderson Romão Jarbas Magalhães Resende Maria Elena de Lima Márcia Helena Borges Rosy Iara Maciel de Azambuja Ribeiro Encapsulated LyeTx III Peptide: Cytotoxic Agent Isolated from <i>Lycosa erythrognatha</i> Spider Venom Toxins antimicrobial peptides antitumor activity spider venom encapsulated peptide |
| title | Encapsulated LyeTx III Peptide: Cytotoxic Agent Isolated from <i>Lycosa erythrognatha</i> Spider Venom |
| title_full | Encapsulated LyeTx III Peptide: Cytotoxic Agent Isolated from <i>Lycosa erythrognatha</i> Spider Venom |
| title_fullStr | Encapsulated LyeTx III Peptide: Cytotoxic Agent Isolated from <i>Lycosa erythrognatha</i> Spider Venom |
| title_full_unstemmed | Encapsulated LyeTx III Peptide: Cytotoxic Agent Isolated from <i>Lycosa erythrognatha</i> Spider Venom |
| title_short | Encapsulated LyeTx III Peptide: Cytotoxic Agent Isolated from <i>Lycosa erythrognatha</i> Spider Venom |
| title_sort | encapsulated lyetx iii peptide cytotoxic agent isolated from i lycosa erythrognatha i spider venom |
| topic | antimicrobial peptides antitumor activity spider venom encapsulated peptide |
| url | https://www.mdpi.com/2072-6651/17/1/32 |
| work_keys_str_mv | AT danielmoreiradossantos encapsulatedlyetxiiipeptidecytotoxicagentisolatedfromilycosaerythrognathaispidervenom AT liviaramossantiago encapsulatedlyetxiiipeptidecytotoxicagentisolatedfromilycosaerythrognathaispidervenom AT nayaraaraujodossantos encapsulatedlyetxiiipeptidecytotoxicagentisolatedfromilycosaerythrognathaispidervenom AT wandersonromao encapsulatedlyetxiiipeptidecytotoxicagentisolatedfromilycosaerythrognathaispidervenom AT jarbasmagalhaesresende encapsulatedlyetxiiipeptidecytotoxicagentisolatedfromilycosaerythrognathaispidervenom AT mariaelenadelima encapsulatedlyetxiiipeptidecytotoxicagentisolatedfromilycosaerythrognathaispidervenom AT marciahelenaborges encapsulatedlyetxiiipeptidecytotoxicagentisolatedfromilycosaerythrognathaispidervenom AT rosyiaramacieldeazambujaribeiro encapsulatedlyetxiiipeptidecytotoxicagentisolatedfromilycosaerythrognathaispidervenom |