In vitro Antitumor Activity and Electrochemical Studies of Bio-Electroactive Anthraquinone Derivatives in Glioblastoma

Abstract This study investigated the antiglioblastoma potential of nine synthetic anthraquinone derivatives (SAQDs). SAQDs were tested for their in vitro cytotoxicity against PBMCs and GBM02 cells. For PBMC, SAQDs showed no cytotoxic effects up to 100 µM. Four most promising SAQDs (1, 4, 5, and 9) d...

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Main Authors: SUELLEN MARIA A. DA SILVA, JOÃO KAYCKE S. DA SILVA, JOICE FRAGOSO O. DE ARAÚJO, ANDRESSA LETÍCIA L. DA SILVA, ANDRESA KATHERINE A. DE ALMEIDA, TAMIRES A. DO NASCIMENTO, ANDRÉ FELIPPE A. XAVIER, RENATA G. DE ALMEIDA, GUILHERME AUGUSTI M. JARDIM, EUFRÂNIO N. DA SILVA JÚNIOR, VIVALDO MOURA-NETO, ALYSSON WAGNER F. DUARTE, PIERRE BARNABÉ ESCODRO, JOÃO XAVIER DE ARAÚJO JÚNIOR, MARÍLIA O.F. GOULART, ALINE C. DE QUEIROZ, MAGNA SUZANA ALEXANDRE-MOREIRA
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Language:English
Published: Academia Brasileira de Ciências 2025-06-01
Series:Anais da Academia Brasileira de Ciências
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Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652025000300601&lng=en&tlng=en
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author SUELLEN MARIA A. DA SILVA
JOÃO KAYCKE S. DA SILVA
JOICE FRAGOSO O. DE ARAÚJO
ANDRESSA LETÍCIA L. DA SILVA
ANDRESA KATHERINE A. DE ALMEIDA
TAMIRES A. DO NASCIMENTO
ANDRÉ FELIPPE A. XAVIER
RENATA G. DE ALMEIDA
GUILHERME AUGUSTI M. JARDIM
EUFRÂNIO N. DA SILVA JÚNIOR
VIVALDO MOURA-NETO
ALYSSON WAGNER F. DUARTE
PIERRE BARNABÉ ESCODRO
JOÃO XAVIER DE ARAÚJO JÚNIOR
MARÍLIA O.F. GOULART
ALINE C. DE QUEIROZ
MAGNA SUZANA ALEXANDRE-MOREIRA
author_facet SUELLEN MARIA A. DA SILVA
JOÃO KAYCKE S. DA SILVA
JOICE FRAGOSO O. DE ARAÚJO
ANDRESSA LETÍCIA L. DA SILVA
ANDRESA KATHERINE A. DE ALMEIDA
TAMIRES A. DO NASCIMENTO
ANDRÉ FELIPPE A. XAVIER
RENATA G. DE ALMEIDA
GUILHERME AUGUSTI M. JARDIM
EUFRÂNIO N. DA SILVA JÚNIOR
VIVALDO MOURA-NETO
ALYSSON WAGNER F. DUARTE
PIERRE BARNABÉ ESCODRO
JOÃO XAVIER DE ARAÚJO JÚNIOR
MARÍLIA O.F. GOULART
ALINE C. DE QUEIROZ
MAGNA SUZANA ALEXANDRE-MOREIRA
author_sort SUELLEN MARIA A. DA SILVA
collection DOAJ
description Abstract This study investigated the antiglioblastoma potential of nine synthetic anthraquinone derivatives (SAQDs). SAQDs were tested for their in vitro cytotoxicity against PBMCs and GBM02 cells. For PBMC, SAQDs showed no cytotoxic effects up to 100 µM. Four most promising SAQDs (1, 4, 5, and 9) demonstrated significant activity against GBM02, with selectivity index >4.54 for 1, >1.30 for 4 and 5, and >1.19 for 9. These compounds induced morphological changes, incluing cell rounding, cytoplasmic vacuolation, and membrane rupture, and inhibited cell migration. Due to the pharmacological role of oxidative stress, electrochemical methods were used to compare the reductive and oxidative capacities of the SAQD with their antitumor activity. Considering the reported findings, it is feasible to confirm that some SAQDs have antitumor effects, supporting additional studies of AQ for developing novel anticancer medications. Due to the small number of compounds investigated, it was not possible to observe a relationship between electrochemical and biological data. However, the electrochemical data are new and could be used to prepare other compounds on this line. CV data revealed that the sulfone’s electron-withdrawing effect facilitated the reduction. At the same time, the sulfides had shown less positive potential for oxidation waves due to the presence of electron-donating groups.
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spelling doaj-art-cd911e224e1b4a8398833890f2cf117d2025-08-20T02:39:32ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902025-06-0197310.1590/0001-3765202520241466In vitro Antitumor Activity and Electrochemical Studies of Bio-Electroactive Anthraquinone Derivatives in GlioblastomaSUELLEN MARIA A. DA SILVAhttps://orcid.org/0000-0003-1944-9513JOÃO KAYCKE S. DA SILVAhttps://orcid.org/0000-0002-5962-7858JOICE FRAGOSO O. DE ARAÚJOhttps://orcid.org/0000-0002-8478-1531ANDRESSA LETÍCIA L. DA SILVAhttps://orcid.org/0000-0002-0952-7613ANDRESA KATHERINE A. DE ALMEIDAhttps://orcid.org/0000-0001-9520-7394TAMIRES A. DO NASCIMENTOhttps://orcid.org/0000-0001-9726-8107ANDRÉ FELIPPE A. XAVIERhttps://orcid.org/0009-0000-7387-6001RENATA G. DE ALMEIDAhttps://orcid.org/0000-0003-2896-0487GUILHERME AUGUSTI M. JARDIMhttps://orcid.org/0000-0002-9882-3085EUFRÂNIO N. DA SILVA JÚNIORhttps://orcid.org/0000-0003-1281-5453VIVALDO MOURA-NETOhttps://orcid.org/0000-0002-6266-043XALYSSON WAGNER F. DUARTEhttps://orcid.org/0000-0001-9626-7524PIERRE BARNABÉ ESCODROhttps://orcid.org/0000-0002-9409-660XJOÃO XAVIER DE ARAÚJO JÚNIORhttps://orcid.org/0000-0003-3187-183XMARÍLIA O.F. GOULARThttps://orcid.org/0000-0001-9860-3667ALINE C. DE QUEIROZhttps://orcid.org/0000-0002-6362-2726MAGNA SUZANA ALEXANDRE-MOREIRAhttps://orcid.org/0000-0002-9979-1994Abstract This study investigated the antiglioblastoma potential of nine synthetic anthraquinone derivatives (SAQDs). SAQDs were tested for their in vitro cytotoxicity against PBMCs and GBM02 cells. For PBMC, SAQDs showed no cytotoxic effects up to 100 µM. Four most promising SAQDs (1, 4, 5, and 9) demonstrated significant activity against GBM02, with selectivity index >4.54 for 1, >1.30 for 4 and 5, and >1.19 for 9. These compounds induced morphological changes, incluing cell rounding, cytoplasmic vacuolation, and membrane rupture, and inhibited cell migration. Due to the pharmacological role of oxidative stress, electrochemical methods were used to compare the reductive and oxidative capacities of the SAQD with their antitumor activity. Considering the reported findings, it is feasible to confirm that some SAQDs have antitumor effects, supporting additional studies of AQ for developing novel anticancer medications. Due to the small number of compounds investigated, it was not possible to observe a relationship between electrochemical and biological data. However, the electrochemical data are new and could be used to prepare other compounds on this line. CV data revealed that the sulfone’s electron-withdrawing effect facilitated the reduction. At the same time, the sulfides had shown less positive potential for oxidation waves due to the presence of electron-donating groups.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652025000300601&lng=en&tlng=enanthraquinonescellsglioblastomaelectroactivity
spellingShingle SUELLEN MARIA A. DA SILVA
JOÃO KAYCKE S. DA SILVA
JOICE FRAGOSO O. DE ARAÚJO
ANDRESSA LETÍCIA L. DA SILVA
ANDRESA KATHERINE A. DE ALMEIDA
TAMIRES A. DO NASCIMENTO
ANDRÉ FELIPPE A. XAVIER
RENATA G. DE ALMEIDA
GUILHERME AUGUSTI M. JARDIM
EUFRÂNIO N. DA SILVA JÚNIOR
VIVALDO MOURA-NETO
ALYSSON WAGNER F. DUARTE
PIERRE BARNABÉ ESCODRO
JOÃO XAVIER DE ARAÚJO JÚNIOR
MARÍLIA O.F. GOULART
ALINE C. DE QUEIROZ
MAGNA SUZANA ALEXANDRE-MOREIRA
In vitro Antitumor Activity and Electrochemical Studies of Bio-Electroactive Anthraquinone Derivatives in Glioblastoma
Anais da Academia Brasileira de Ciências
anthraquinones
cells
glioblastoma
electroactivity
title In vitro Antitumor Activity and Electrochemical Studies of Bio-Electroactive Anthraquinone Derivatives in Glioblastoma
title_full In vitro Antitumor Activity and Electrochemical Studies of Bio-Electroactive Anthraquinone Derivatives in Glioblastoma
title_fullStr In vitro Antitumor Activity and Electrochemical Studies of Bio-Electroactive Anthraquinone Derivatives in Glioblastoma
title_full_unstemmed In vitro Antitumor Activity and Electrochemical Studies of Bio-Electroactive Anthraquinone Derivatives in Glioblastoma
title_short In vitro Antitumor Activity and Electrochemical Studies of Bio-Electroactive Anthraquinone Derivatives in Glioblastoma
title_sort in vitro antitumor activity and electrochemical studies of bio electroactive anthraquinone derivatives in glioblastoma
topic anthraquinones
cells
glioblastoma
electroactivity
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652025000300601&lng=en&tlng=en
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