Mammalian Expression Cloning of Nucleic Acid Binding Proteins by Agarose Thin-Layer Gelshift Clone Selection
Here we report a functional screening technique to identify cDNAs encoding mammalian nucleic acid binding proteins. We have combined cDNA expression cloning with the agarose thin-layer gelshift assay technique to detect specific nucleic acid binding proteins from a mammalian expression library. We d...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2002-10-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/02334rr03 |
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