In Silico and In Vitro Characterization of <i>Bacillus velezensis</i> P45: Screening for a Novel Probiotic Candidate
Spore-forming <i>Bacilli</i> have been explored due to their potential biotechnological features and applications in human health and functional food research. This study focuses on the genetic and phenotypical characterization of the functional probiotic properties of <i>Bacillus...
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MDPI AG
2025-06-01
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| Series: | Foods |
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| author | Carolini Esmeriz da Rosa Cristian Mauricio Barreto Pinilla Luiza Dalpiccoli Toss Adriano Brandelli |
| author_facet | Carolini Esmeriz da Rosa Cristian Mauricio Barreto Pinilla Luiza Dalpiccoli Toss Adriano Brandelli |
| author_sort | Carolini Esmeriz da Rosa |
| collection | DOAJ |
| description | Spore-forming <i>Bacilli</i> have been explored due to their potential biotechnological features and applications in human health and functional food research. This study focuses on the genetic and phenotypical characterization of the functional probiotic properties of <i>Bacillus velezensis</i> P45, a strain isolated from fish intestines. <i>B. velezensis</i> P45 exhibited antimicrobial activity against Gram-positive and Gram-negative pathogens and demonstrated strong autoaggregation and biofilm formation properties in vitro. The strain also showed tolerance to gastrointestinal conditions and ability to metabolize and adhere to mucin. In silico analysis confirmed the absence of virulence factors and antibiotic resistance genes, reinforcing its safety as a probiotic candidate. Genome mining revealed the presence of genes related to adhesion, such as fibronectin-binding protein and enolases, and for the synthesis of secondary metabolites, including the antimicrobial lipopeptides fengycin, surfactin, and bacillibactin. In addition, phylogenetic comparison using the <i>yloA</i> (<i>rqcH</i>) gene associated with gut adhesion clustered strain P45 with other probiotic <i>Bacillus</i> and <i>B. velezensis</i> strains, while separating it from pathogenic bacteria. Thus, the strain <i>B. velezensis</i> P45 could be a valuable candidate as a probiotic due to its functional properties and safety. |
| format | Article |
| id | doaj-art-cbe12c8eb8184cf1a96cff0032db9bb0 |
| institution | DOAJ |
| issn | 2304-8158 |
| language | English |
| publishDate | 2025-06-01 |
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| spelling | doaj-art-cbe12c8eb8184cf1a96cff0032db9bb02025-08-20T03:16:42ZengMDPI AGFoods2304-81582025-06-011413233410.3390/foods14132334In Silico and In Vitro Characterization of <i>Bacillus velezensis</i> P45: Screening for a Novel Probiotic CandidateCarolini Esmeriz da Rosa0Cristian Mauricio Barreto Pinilla1Luiza Dalpiccoli Toss2Adriano Brandelli3Laboratory of Nanobiotechnology and Applied Microbiology, Department of Food Science, Federal University of Rio Grande do Sul, Porto Alegre 91501-970, BrazilDairy Technology Center, Institute of Food Technology, Campinas 13083-015, BrazilLaboratory of Nanobiotechnology and Applied Microbiology, Department of Food Science, Federal University of Rio Grande do Sul, Porto Alegre 91501-970, BrazilLaboratory of Nanobiotechnology and Applied Microbiology, Department of Food Science, Federal University of Rio Grande do Sul, Porto Alegre 91501-970, BrazilSpore-forming <i>Bacilli</i> have been explored due to their potential biotechnological features and applications in human health and functional food research. This study focuses on the genetic and phenotypical characterization of the functional probiotic properties of <i>Bacillus velezensis</i> P45, a strain isolated from fish intestines. <i>B. velezensis</i> P45 exhibited antimicrobial activity against Gram-positive and Gram-negative pathogens and demonstrated strong autoaggregation and biofilm formation properties in vitro. The strain also showed tolerance to gastrointestinal conditions and ability to metabolize and adhere to mucin. In silico analysis confirmed the absence of virulence factors and antibiotic resistance genes, reinforcing its safety as a probiotic candidate. Genome mining revealed the presence of genes related to adhesion, such as fibronectin-binding protein and enolases, and for the synthesis of secondary metabolites, including the antimicrobial lipopeptides fengycin, surfactin, and bacillibactin. In addition, phylogenetic comparison using the <i>yloA</i> (<i>rqcH</i>) gene associated with gut adhesion clustered strain P45 with other probiotic <i>Bacillus</i> and <i>B. velezensis</i> strains, while separating it from pathogenic bacteria. Thus, the strain <i>B. velezensis</i> P45 could be a valuable candidate as a probiotic due to its functional properties and safety.https://www.mdpi.com/2304-8158/14/13/2334antimicrobial peptides<i>Bacillus</i>genome analysisgut adhesionprobiotics |
| spellingShingle | Carolini Esmeriz da Rosa Cristian Mauricio Barreto Pinilla Luiza Dalpiccoli Toss Adriano Brandelli In Silico and In Vitro Characterization of <i>Bacillus velezensis</i> P45: Screening for a Novel Probiotic Candidate Foods antimicrobial peptides <i>Bacillus</i> genome analysis gut adhesion probiotics |
| title | In Silico and In Vitro Characterization of <i>Bacillus velezensis</i> P45: Screening for a Novel Probiotic Candidate |
| title_full | In Silico and In Vitro Characterization of <i>Bacillus velezensis</i> P45: Screening for a Novel Probiotic Candidate |
| title_fullStr | In Silico and In Vitro Characterization of <i>Bacillus velezensis</i> P45: Screening for a Novel Probiotic Candidate |
| title_full_unstemmed | In Silico and In Vitro Characterization of <i>Bacillus velezensis</i> P45: Screening for a Novel Probiotic Candidate |
| title_short | In Silico and In Vitro Characterization of <i>Bacillus velezensis</i> P45: Screening for a Novel Probiotic Candidate |
| title_sort | in silico and in vitro characterization of i bacillus velezensis i p45 screening for a novel probiotic candidate |
| topic | antimicrobial peptides <i>Bacillus</i> genome analysis gut adhesion probiotics |
| url | https://www.mdpi.com/2304-8158/14/13/2334 |
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