Physicochemical and functional assessment of messenger RNA 5′Cap-end impurities under forced degradation conditions

Physicochemical and functional assessment of messenger RNA 5′Cap-end impurities under forced degradation conditions

The 5′Cap is a modified nucleotide structure added to the 5′end of mRNA, which plays a critical role in stability and translation of the molecule. Accurate characterization of 5′Cap impurities is crucial during development of mRNA therapeutics. Here, we utilized orthogonal liquid chromatography-mass...

Full description

Saved in:
Bibliographic Details
Main Authors: J. Hutchinson, F. Schweikart, R. Shannon, A. Murthy, S.M. Bates, S. Leone, T. Nissan, D. Sideris, C. Lal, J. Button, R. Rani, G. Thom, N. Bond, E. Örnskov, S. Trabulo
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Molecular Therapy: Nucleic Acids
Subjects:
MT: oligonucleotides: therapies and applications
RNA
5′Cap
liquid chromatography-mass spectrometry
critical quality attributes
forced degradation
Online Access:http://www.sciencedirect.com/science/article/pii/S2162253125001246
Tags: Add Tag
No Tags, Be the first to tag this record!

Internet

http://www.sciencedirect.com/science/article/pii/S2162253125001246

Similar Items