Expression of β-glucanase Gene from Bacillus macerans in Escherichia coli
The β-glucanase gene from recombined cloning plasmid was subcloned into BamH Ⅰ and Xho Ⅰsite of expression plasmid pET-30a. The recombined expression plasmid was transformed into Escherichia coli strain BL21. The result of SDS-PAGE showed that about 25.0 kD protein of β-1, 3-1,4-glucanase was expres...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2005-09-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2005.05.0628 |
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| author | LI Wei-fen YAO Jiang-tao HU Chun-xia XU Zi-rong |
| author_facet | LI Wei-fen YAO Jiang-tao HU Chun-xia XU Zi-rong |
| author_sort | LI Wei-fen |
| collection | DOAJ |
| description | The β-glucanase gene from recombined cloning plasmid was subcloned into BamH Ⅰ and Xho Ⅰsite of expression plasmid pET-30a. The recombined expression plasmid was transformed into Escherichia coli strain BL21. The result of SDS-PAGE showed that about 25.0 kD protein of β-1, 3-1,4-glucanase was expressed in E. coli strain BL21 containing recombined plasmid. The enzyme activity of the recombinant was 60.4 U/mL, which was about 30-fold higher than that of the original strain. The optimal temperature and pH of the enzyme were 50℃ and 5.0-7.0, respectively. The enzyme was relatively stable at temperature below 50℃ for 2 min, and almost unaffected after 60min between pH 3.0 and 9.0. |
| format | Article |
| id | doaj-art-caf86e76f8c8444a922b09983aa7211f |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2005-09-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-caf86e76f8c8444a922b09983aa7211f2025-08-20T03:58:19ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552005-09-013162863210.3785/1008-9209.2005.05.062810089209Expression of β-glucanase Gene from Bacillus macerans in Escherichia coliLI Wei-fenYAO Jiang-taoHU Chun-xiaXU Zi-rongThe β-glucanase gene from recombined cloning plasmid was subcloned into BamH Ⅰ and Xho Ⅰsite of expression plasmid pET-30a. The recombined expression plasmid was transformed into Escherichia coli strain BL21. The result of SDS-PAGE showed that about 25.0 kD protein of β-1, 3-1,4-glucanase was expressed in E. coli strain BL21 containing recombined plasmid. The enzyme activity of the recombinant was 60.4 U/mL, which was about 30-fold higher than that of the original strain. The optimal temperature and pH of the enzyme were 50℃ and 5.0-7.0, respectively. The enzyme was relatively stable at temperature below 50℃ for 2 min, and almost unaffected after 60min between pH 3.0 and 9.0.https://www.academax.com/doi/10.3785/1008-9209.2005.05.0628β-glucanase<italic>Bacillus macerans</italic><italic>Escherichia coli</italic>expressionenzymatic properties |
| spellingShingle | LI Wei-fen YAO Jiang-tao HU Chun-xia XU Zi-rong Expression of β-glucanase Gene from Bacillus macerans in Escherichia coli 浙江大学学报. 农业与生命科学版 β-glucanase <italic>Bacillus macerans</italic> <italic>Escherichia coli</italic> expression enzymatic properties |
| title | Expression of β-glucanase Gene from Bacillus macerans in Escherichia coli |
| title_full | Expression of β-glucanase Gene from Bacillus macerans in Escherichia coli |
| title_fullStr | Expression of β-glucanase Gene from Bacillus macerans in Escherichia coli |
| title_full_unstemmed | Expression of β-glucanase Gene from Bacillus macerans in Escherichia coli |
| title_short | Expression of β-glucanase Gene from Bacillus macerans in Escherichia coli |
| title_sort | expression of β glucanase gene from bacillus macerans in escherichia coli |
| topic | β-glucanase <italic>Bacillus macerans</italic> <italic>Escherichia coli</italic> expression enzymatic properties |
| url | https://www.academax.com/doi/10.3785/1008-9209.2005.05.0628 |
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