Expression of β-glucanase Gene from Bacillus macerans in Escherichia coli

The β-glucanase gene from recombined cloning plasmid was subcloned into BamH Ⅰ and Xho Ⅰsite of expression plasmid pET-30a. The recombined expression plasmid was transformed into Escherichia coli strain BL21. The result of SDS-PAGE showed that about 25.0 kD protein of β-1, 3-1,4-glucanase was expres...

Full description

Saved in:
Bibliographic Details
Main Authors: LI Wei-fen, YAO Jiang-tao, HU Chun-xia, XU Zi-rong
Format: Article
Language:English
Published: Zhejiang University Press 2005-09-01
Series:浙江大学学报. 农业与生命科学版
Subjects:
Online Access:https://www.academax.com/doi/10.3785/1008-9209.2005.05.0628
Tags: Add Tag
No Tags, Be the first to tag this record!