Expression of β-glucanase Gene from Bacillus macerans in Escherichia coli
The β-glucanase gene from recombined cloning plasmid was subcloned into BamH Ⅰ and Xho Ⅰsite of expression plasmid pET-30a. The recombined expression plasmid was transformed into Escherichia coli strain BL21. The result of SDS-PAGE showed that about 25.0 kD protein of β-1, 3-1,4-glucanase was expres...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2005-09-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2005.05.0628 |
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| Summary: | The β-glucanase gene from recombined cloning plasmid was subcloned into BamH Ⅰ and Xho Ⅰsite of expression plasmid pET-30a. The recombined expression plasmid was transformed into Escherichia coli strain BL21. The result of SDS-PAGE showed that about 25.0 kD protein of β-1, 3-1,4-glucanase was expressed in E. coli strain BL21 containing recombined plasmid. The enzyme activity of the recombinant was 60.4 U/mL, which was about 30-fold higher than that of the original strain. The optimal temperature and pH of the enzyme were 50℃ and 5.0-7.0, respectively. The enzyme was relatively stable at temperature below 50℃ for 2 min, and almost unaffected after 60min between pH 3.0 and 9.0. |
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| ISSN: | 1008-9209 2097-5155 |