High-resolution mass spectrometry measurement of N-terminal carbamylated hemoglobin as a potential marker for chronic diseases with elevated blood urea levels
Introduction: N-terminal carbamylated hemoglobin (CarHb) reflects long-term blood urea levels and has potential as a marker for chronic kidney disease (CKD) and other chronic conditions with elevated blood urea levels. A liquid chromatography-high-resolution mass spectrometry (LC-HR-MS) method was d...
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Elsevier
2025-01-01
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| Series: | Journal of Mass Spectrometry and Advances in the Clinical Lab |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667145X2500001X |
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| author | Fangjun Chen Priscilla S.-W. Yeung Carolyn V. Wong Ruben Y. Luo |
| author_facet | Fangjun Chen Priscilla S.-W. Yeung Carolyn V. Wong Ruben Y. Luo |
| author_sort | Fangjun Chen |
| collection | DOAJ |
| description | Introduction: N-terminal carbamylated hemoglobin (CarHb) reflects long-term blood urea levels and has potential as a marker for chronic kidney disease (CKD) and other chronic conditions with elevated blood urea levels. A liquid chromatography-high-resolution mass spectrometry (LC-HR-MS) method was developed to measure CarHb. Methods: Apparent CarHb/Hb ratios were calculated from the peak area ratios of carbamylated to native N-terminal peptides digested from hemoglobin alpha and beta subunits. Blood samples from healthy individuals, CKD patients, and chronic obstructive pulmonary disease (COPD) patients were analyzed. Results: The apparent CarHb/Hb ratios were significantly higher in CKD and COPD patients compared to healthy individuals. However, no significant differences were observed between the CKD and COPD patient groups. Conclusions: In this study, an LC-HR-MS method was developed for quantifying the apparent CarHb/Hb ratios and exploring their potential for clinical diagnostic applications. CarHb is a promising marker for monitoring kidney diseases and other chronic conditions with elevated blood urea levels. Beyond CarHb, the use of other carbamylated proteins as clinical diagnostic and prognostic markers can be explored. |
| format | Article |
| id | doaj-art-ca1161ac4d1f4215a266e4fe9fdad867 |
| institution | Kabale University |
| issn | 2667-145X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Mass Spectrometry and Advances in the Clinical Lab |
| spelling | doaj-art-ca1161ac4d1f4215a266e4fe9fdad8672025-02-09T05:01:39ZengElsevierJournal of Mass Spectrometry and Advances in the Clinical Lab2667-145X2025-01-0135813High-resolution mass spectrometry measurement of N-terminal carbamylated hemoglobin as a potential marker for chronic diseases with elevated blood urea levelsFangjun Chen0Priscilla S.-W. Yeung1Carolyn V. Wong2Ruben Y. Luo3Department of Pathology, Stanford University, Stanford, CA, USA; Clinical Laboratories, Stanford Health Care, Palo Alto, CA, USA; Department of Laboratory Medicine, Zhongshan Hospital, Fudan University, Shanghai, ChinaDepartment of Pathology, Stanford University, Stanford, CA, USA; Clinical Laboratories, Stanford Health Care, Palo Alto, CA, USAClinical Laboratories, Stanford Health Care, Palo Alto, CA, USADepartment of Pathology, Stanford University, Stanford, CA, USA; Clinical Laboratories, Stanford Health Care, Palo Alto, CA, USA; Corresponding author at: 3375 Hillview Ave, Palo Alto, CA 94304, USA.Introduction: N-terminal carbamylated hemoglobin (CarHb) reflects long-term blood urea levels and has potential as a marker for chronic kidney disease (CKD) and other chronic conditions with elevated blood urea levels. A liquid chromatography-high-resolution mass spectrometry (LC-HR-MS) method was developed to measure CarHb. Methods: Apparent CarHb/Hb ratios were calculated from the peak area ratios of carbamylated to native N-terminal peptides digested from hemoglobin alpha and beta subunits. Blood samples from healthy individuals, CKD patients, and chronic obstructive pulmonary disease (COPD) patients were analyzed. Results: The apparent CarHb/Hb ratios were significantly higher in CKD and COPD patients compared to healthy individuals. However, no significant differences were observed between the CKD and COPD patient groups. Conclusions: In this study, an LC-HR-MS method was developed for quantifying the apparent CarHb/Hb ratios and exploring their potential for clinical diagnostic applications. CarHb is a promising marker for monitoring kidney diseases and other chronic conditions with elevated blood urea levels. Beyond CarHb, the use of other carbamylated proteins as clinical diagnostic and prognostic markers can be explored.http://www.sciencedirect.com/science/article/pii/S2667145X2500001XHigh-resolution mass spectrometryCarbamylated hemoglobinChronic diseaseBlood urea |
| spellingShingle | Fangjun Chen Priscilla S.-W. Yeung Carolyn V. Wong Ruben Y. Luo High-resolution mass spectrometry measurement of N-terminal carbamylated hemoglobin as a potential marker for chronic diseases with elevated blood urea levels Journal of Mass Spectrometry and Advances in the Clinical Lab High-resolution mass spectrometry Carbamylated hemoglobin Chronic disease Blood urea |
| title | High-resolution mass spectrometry measurement of N-terminal carbamylated hemoglobin as a potential marker for chronic diseases with elevated blood urea levels |
| title_full | High-resolution mass spectrometry measurement of N-terminal carbamylated hemoglobin as a potential marker for chronic diseases with elevated blood urea levels |
| title_fullStr | High-resolution mass spectrometry measurement of N-terminal carbamylated hemoglobin as a potential marker for chronic diseases with elevated blood urea levels |
| title_full_unstemmed | High-resolution mass spectrometry measurement of N-terminal carbamylated hemoglobin as a potential marker for chronic diseases with elevated blood urea levels |
| title_short | High-resolution mass spectrometry measurement of N-terminal carbamylated hemoglobin as a potential marker for chronic diseases with elevated blood urea levels |
| title_sort | high resolution mass spectrometry measurement of n terminal carbamylated hemoglobin as a potential marker for chronic diseases with elevated blood urea levels |
| topic | High-resolution mass spectrometry Carbamylated hemoglobin Chronic disease Blood urea |
| url | http://www.sciencedirect.com/science/article/pii/S2667145X2500001X |
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