Infiltration and subtype analysis of CD3 + CD20 + T cells in lung cancer
Abstract Background CD3 + CD20 + T cells (TB cells) are a subset of lymphocytes in the human body that are associated with inflammation. They originate from T cells interacting with B cells, and their levels are abnormally elevated in individuals with immune disorders, as well as in some cancer pati...
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BMC
2025-01-01
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| Series: | BMC Cancer |
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| Online Access: | https://doi.org/10.1186/s12885-025-13581-7 |
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| author | Liping Luo Juan Ji Juan Dong Maotao He Wenjun Jiang Yang Liu Weidong Wang |
| author_facet | Liping Luo Juan Ji Juan Dong Maotao He Wenjun Jiang Yang Liu Weidong Wang |
| author_sort | Liping Luo |
| collection | DOAJ |
| description | Abstract Background CD3 + CD20 + T cells (TB cells) are a subset of lymphocytes in the human body that are associated with inflammation. They originate from T cells interacting with B cells, and their levels are abnormally elevated in individuals with immune disorders, as well as in some cancer patients. The interplay between tumor immunity and inflammation is intricate, yet the specific involvement of TB cells in local tumor immunity remains uncertain, with limited research on their subtypes. Methods Lung cancer surgical samples were stained using multi-color immunofluorescence to study the subtypes and distribution patterns of TB cells. Results TB cells were confirmed to exist in a scattered pattern within tertiary lymphoid structures (TLS) in lung cancer tissues, with higher abundance in mature TLS. In subtype analysis, the CD4-CD8- double-negative TB cell subtype was predominant, comprising over 90% in samples with abundant TLS infiltration and over 60% in samples with poor infiltration. This was followed by the CD4 + CD8- and CD4-CD8 + single-positive TB cell subtypes, while the CD4 + CD8 + double-positive TB cell subtype was nearly absent. During the maturation of TLS, the proportion of B cells gradually increased, while the proportion of CD4-CD8- T cell subtype decreased. Conclusions TB cells extensively infiltrate the TLS regions in tumor tissues, with the double-negative subtype being predominant, potentially playing a crucial regulatory role in the local tumor immune microenvironment. This finding could facilitate the advancement of novel cancer treatment strategies. |
| format | Article |
| id | doaj-art-c9a9eab9451f4c8fb05b41fe3541a541 |
| institution | Kabale University |
| issn | 1471-2407 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj-art-c9a9eab9451f4c8fb05b41fe3541a5412025-02-02T12:28:44ZengBMCBMC Cancer1471-24072025-01-012511810.1186/s12885-025-13581-7Infiltration and subtype analysis of CD3 + CD20 + T cells in lung cancerLiping Luo0Juan Ji1Juan Dong2Maotao He3Wenjun Jiang4Yang Liu5Weidong Wang6Basic Research Center, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Center, School of Medicine, Sichuan Cancer Hospital & Institute, University of Electronic Science and Technology of ChinaPathology Department, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Hospital & Institute, Affiliated Cancer Hospital of University of Electronic Science and Technology of ChinaDepartment of Pulmonology, Meishan Cancer HospitalPathology Department, Meishan Cancer HospitalBasic Research Center, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Center, School of Medicine, Sichuan Cancer Hospital & Institute, University of Electronic Science and Technology of ChinaPathology Department, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Hospital & Institute, Affiliated Cancer Hospital of University of Electronic Science and Technology of ChinaBasic Research Center, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Center, School of Medicine, Sichuan Cancer Hospital & Institute, University of Electronic Science and Technology of ChinaAbstract Background CD3 + CD20 + T cells (TB cells) are a subset of lymphocytes in the human body that are associated with inflammation. They originate from T cells interacting with B cells, and their levels are abnormally elevated in individuals with immune disorders, as well as in some cancer patients. The interplay between tumor immunity and inflammation is intricate, yet the specific involvement of TB cells in local tumor immunity remains uncertain, with limited research on their subtypes. Methods Lung cancer surgical samples were stained using multi-color immunofluorescence to study the subtypes and distribution patterns of TB cells. Results TB cells were confirmed to exist in a scattered pattern within tertiary lymphoid structures (TLS) in lung cancer tissues, with higher abundance in mature TLS. In subtype analysis, the CD4-CD8- double-negative TB cell subtype was predominant, comprising over 90% in samples with abundant TLS infiltration and over 60% in samples with poor infiltration. This was followed by the CD4 + CD8- and CD4-CD8 + single-positive TB cell subtypes, while the CD4 + CD8 + double-positive TB cell subtype was nearly absent. During the maturation of TLS, the proportion of B cells gradually increased, while the proportion of CD4-CD8- T cell subtype decreased. Conclusions TB cells extensively infiltrate the TLS regions in tumor tissues, with the double-negative subtype being predominant, potentially playing a crucial regulatory role in the local tumor immune microenvironment. This finding could facilitate the advancement of novel cancer treatment strategies.https://doi.org/10.1186/s12885-025-13581-7Lung cancerTertiary lymphoid structuresCD3 + CD20 + T cellDouble negative T cell |
| spellingShingle | Liping Luo Juan Ji Juan Dong Maotao He Wenjun Jiang Yang Liu Weidong Wang Infiltration and subtype analysis of CD3 + CD20 + T cells in lung cancer BMC Cancer Lung cancer Tertiary lymphoid structures CD3 + CD20 + T cell Double negative T cell |
| title | Infiltration and subtype analysis of CD3 + CD20 + T cells in lung cancer |
| title_full | Infiltration and subtype analysis of CD3 + CD20 + T cells in lung cancer |
| title_fullStr | Infiltration and subtype analysis of CD3 + CD20 + T cells in lung cancer |
| title_full_unstemmed | Infiltration and subtype analysis of CD3 + CD20 + T cells in lung cancer |
| title_short | Infiltration and subtype analysis of CD3 + CD20 + T cells in lung cancer |
| title_sort | infiltration and subtype analysis of cd3 cd20 t cells in lung cancer |
| topic | Lung cancer Tertiary lymphoid structures CD3 + CD20 + T cell Double negative T cell |
| url | https://doi.org/10.1186/s12885-025-13581-7 |
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