Promoting effects of NaCl and KCl stresses on astaxanthin yield in Microcystis flos-aquae
This study was to uncover the astaxanthin accumulation mechanism of Microcystis flos-aquae stressed by NaCl and KCl and select the optimal condition for astaxanthin production. Both of NaCl and KCl stresses showed inhibiting effects on M. flos-aquae growth by reducing photosynthetic abilities and ca...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-04-01
|
| Series: | Food Chemistry: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157525002895 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849321395314491392 |
|---|---|
| author | Junjie Ma Zhehan Yang Zhuxin Jin Lexin Huang Yinggang Wei Wangbo Chen Zhaojiang Zuo |
| author_facet | Junjie Ma Zhehan Yang Zhuxin Jin Lexin Huang Yinggang Wei Wangbo Chen Zhaojiang Zuo |
| author_sort | Junjie Ma |
| collection | DOAJ |
| description | This study was to uncover the astaxanthin accumulation mechanism of Microcystis flos-aquae stressed by NaCl and KCl and select the optimal condition for astaxanthin production. Both of NaCl and KCl stresses showed inhibiting effects on M. flos-aquae growth by reducing photosynthetic abilities and causing reactive oxygen species accumulation. With raising the two salt concentrations, astaxanthin content and yield gradually increased, and the highest accumulation was under 300 mM for each salt, which should result from the up-regulation of 6 related genes promoting the precursor (β-carotene and zeaxanthin) transformation. KCl stress was more effective for improving astaxanthin yield than NaCl stress, which was strongly related with the salt concentration and astaxanthin content. Compared with other potential suitable conditions (35°C and purple light), 300 mM KCl also exhibited maximum effect on astaxanthin accumulation. Therefore, M. flos-aquae is first identified to synthesize astaxanthin, and KCl stress is more favorable to the compound production. |
| format | Article |
| id | doaj-art-c98ba12ffab545d89fd9ad1e19cde614 |
| institution | Kabale University |
| issn | 2590-1575 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-c98ba12ffab545d89fd9ad1e19cde6142025-08-20T03:49:45ZengElsevierFood Chemistry: X2590-15752025-04-012710244210.1016/j.fochx.2025.102442Promoting effects of NaCl and KCl stresses on astaxanthin yield in Microcystis flos-aquaeJunjie Ma0Zhehan Yang1Zhuxin Jin2Lexin Huang3Yinggang Wei4Wangbo Chen5Zhaojiang Zuo6National Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou 311300, China; College of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, ChinaNational Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou 311300, China; College of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, ChinaNational Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou 311300, China; College of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, ChinaNational Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou 311300, China; College of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, ChinaNational Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou 311300, China; College of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, ChinaNational Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou 311300, China; College of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, ChinaNational Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou 311300, China; College of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, China; Corresponding author at: National Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou 311300, China.This study was to uncover the astaxanthin accumulation mechanism of Microcystis flos-aquae stressed by NaCl and KCl and select the optimal condition for astaxanthin production. Both of NaCl and KCl stresses showed inhibiting effects on M. flos-aquae growth by reducing photosynthetic abilities and causing reactive oxygen species accumulation. With raising the two salt concentrations, astaxanthin content and yield gradually increased, and the highest accumulation was under 300 mM for each salt, which should result from the up-regulation of 6 related genes promoting the precursor (β-carotene and zeaxanthin) transformation. KCl stress was more effective for improving astaxanthin yield than NaCl stress, which was strongly related with the salt concentration and astaxanthin content. Compared with other potential suitable conditions (35°C and purple light), 300 mM KCl also exhibited maximum effect on astaxanthin accumulation. Therefore, M. flos-aquae is first identified to synthesize astaxanthin, and KCl stress is more favorable to the compound production.http://www.sciencedirect.com/science/article/pii/S2590157525002895AstaxanthinGene expressionMicrocystis flos-aquaeOptimal conditionSalt stress |
| spellingShingle | Junjie Ma Zhehan Yang Zhuxin Jin Lexin Huang Yinggang Wei Wangbo Chen Zhaojiang Zuo Promoting effects of NaCl and KCl stresses on astaxanthin yield in Microcystis flos-aquae Food Chemistry: X Astaxanthin Gene expression Microcystis flos-aquae Optimal condition Salt stress |
| title | Promoting effects of NaCl and KCl stresses on astaxanthin yield in Microcystis flos-aquae |
| title_full | Promoting effects of NaCl and KCl stresses on astaxanthin yield in Microcystis flos-aquae |
| title_fullStr | Promoting effects of NaCl and KCl stresses on astaxanthin yield in Microcystis flos-aquae |
| title_full_unstemmed | Promoting effects of NaCl and KCl stresses on astaxanthin yield in Microcystis flos-aquae |
| title_short | Promoting effects of NaCl and KCl stresses on astaxanthin yield in Microcystis flos-aquae |
| title_sort | promoting effects of nacl and kcl stresses on astaxanthin yield in microcystis flos aquae |
| topic | Astaxanthin Gene expression Microcystis flos-aquae Optimal condition Salt stress |
| url | http://www.sciencedirect.com/science/article/pii/S2590157525002895 |
| work_keys_str_mv | AT junjiema promotingeffectsofnaclandkclstressesonastaxanthinyieldinmicrocystisflosaquae AT zhehanyang promotingeffectsofnaclandkclstressesonastaxanthinyieldinmicrocystisflosaquae AT zhuxinjin promotingeffectsofnaclandkclstressesonastaxanthinyieldinmicrocystisflosaquae AT lexinhuang promotingeffectsofnaclandkclstressesonastaxanthinyieldinmicrocystisflosaquae AT yinggangwei promotingeffectsofnaclandkclstressesonastaxanthinyieldinmicrocystisflosaquae AT wangbochen promotingeffectsofnaclandkclstressesonastaxanthinyieldinmicrocystisflosaquae AT zhaojiangzuo promotingeffectsofnaclandkclstressesonastaxanthinyieldinmicrocystisflosaquae |