A long shelf-life melon created via CRISPR/Cas9 RNP-based in planta genome editing

Genome editing in melon (Cucumis melo L.) remains a significant challenge due to the inefficiencies associated with conventional cell culture-based transformation methods. In the present study, a novel in planta Particle Bombardment (iPB) approach was developed to enable DNA-free genome editing in m...

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Bibliographic Details
Main Authors: Kentaro Sasaki, Kaoru Urano, Naozumi Mimida, Satoko Nonaka, Hiroshi Ezura, Ryozo Imai
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-06-01
Series:Frontiers in Genome Editing
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Online Access:https://www.frontiersin.org/articles/10.3389/fgeed.2025.1623097/full
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