Comparison of active site mutations at subsite + 2 of Anoxybacillus ayderensis A9 β-glucosidase for hydrolysis of pNPG and polydatin
Abstract β-glucosidase from Anoxybacillus ayderensis A9 (BglA9) is a potent enzyme for enzymatic hydrolysis of polydatin to resveratrol. Based on structural and bioinformatics analysis an area near + 2 subsite of the active site pocket of BglA9 was selected and single point mutations were introduced...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-07-01
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| Series: | BMC Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12896-025-00984-4 |
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