Comparison of active site mutations at subsite + 2 of Anoxybacillus ayderensis A9 β-glucosidase for hydrolysis of pNPG and polydatin

Comparison of active site mutations at subsite + 2 of Anoxybacillus ayderensis A9 β-glucosidase for hydrolysis of pNPG and polydatin

Abstract β-glucosidase from Anoxybacillus ayderensis A9 (BglA9) is a potent enzyme for enzymatic hydrolysis of polydatin to resveratrol. Based on structural and bioinformatics analysis an area near + 2 subsite of the active site pocket of BglA9 was selected and single point mutations were introduced...

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Main Authors: Numan Saleh Zada, Ali Osman Belduz, Abdulrahman H. Alessa, Halil Ibrahim Güler, Mine Karaoğlan, Malik Badshah, Aamer Ali Shah, Aasia Kalsoom, Samiullah Khan
Format: Article
Language:English
Published: BMC 2025-07-01
Series:BMC Biotechnology
Subjects:
Β-glucosidase
Polydatin
Anoxybacillus ayderensis A9
Active site mutations
Rational designing
Online Access:https://doi.org/10.1186/s12896-025-00984-4
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https://doi.org/10.1186/s12896-025-00984-4

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