A versatile and efficient method to isolate nuclei from low-input cryopreserved tissues for single-nuclei transcriptomics

A versatile and efficient method to isolate nuclei from low-input cryopreserved tissues for single-nuclei transcriptomics

Abstract Clinical samples are vital for understanding diseases, but their scarcity requires refined research methods. Emerging single-cell technologies offer detailed views of tissue heterogeneity but need sufficient fully characterized tissues. We developed an optimized single-nuclei RNA sequencing...

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Main Authors: Cristopher Segovia, Vincent Desrosiers, Fatemeh Khadangi, Karine Robitaille, Victoria Saavedra Armero, Myreille D’Astous, Gabriel Khelifi, Alain Bergeron, Samer Hussein, Maxime Richer, Yohan Bossé, Yves Fradet, Vincent Fradet, Steve Bilodeau
Format: Article
Language:English
Published: Nature Portfolio 2025-02-01
Series:Scientific Reports
Subjects:
Nuclei isolation
Tissue heterogeneity
Transcriptomics
Cancer
Tissue homogenization
Nuclei sorting
Online Access:https://doi.org/10.1038/s41598-025-90070-8
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https://doi.org/10.1038/s41598-025-90070-8

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