Influenza virus drug resistance: a time-sampled population genetics perspective.

The challenge of distinguishing genetic drift from selection remains a central focus of population genetics. Time-sampled data may provide a powerful tool for distinguishing these processes, and we here propose approximate Bayesian, maximum likelihood, and analytical methods for the inference of dem...

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Main Authors: Matthieu Foll, Yu-Ping Poh, Nicholas Renzette, Anna Ferrer-Admetlla, Claudia Bank, Hyunjin Shim, Anna-Sapfo Malaspinas, Gregory Ewing, Ping Liu, Daniel Wegmann, Daniel R Caffrey, Konstantin B Zeldovich, Daniel N Bolon, Jennifer P Wang, Timothy F Kowalik, Celia A Schiffer, Robert W Finberg, Jeffrey D Jensen
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-02-01
Series:PLoS Genetics
Online Access:https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1004185&type=printable
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author Matthieu Foll
Yu-Ping Poh
Nicholas Renzette
Anna Ferrer-Admetlla
Claudia Bank
Hyunjin Shim
Anna-Sapfo Malaspinas
Gregory Ewing
Ping Liu
Daniel Wegmann
Daniel R Caffrey
Konstantin B Zeldovich
Daniel N Bolon
Jennifer P Wang
Timothy F Kowalik
Celia A Schiffer
Robert W Finberg
Jeffrey D Jensen
author_facet Matthieu Foll
Yu-Ping Poh
Nicholas Renzette
Anna Ferrer-Admetlla
Claudia Bank
Hyunjin Shim
Anna-Sapfo Malaspinas
Gregory Ewing
Ping Liu
Daniel Wegmann
Daniel R Caffrey
Konstantin B Zeldovich
Daniel N Bolon
Jennifer P Wang
Timothy F Kowalik
Celia A Schiffer
Robert W Finberg
Jeffrey D Jensen
author_sort Matthieu Foll
collection DOAJ
description The challenge of distinguishing genetic drift from selection remains a central focus of population genetics. Time-sampled data may provide a powerful tool for distinguishing these processes, and we here propose approximate Bayesian, maximum likelihood, and analytical methods for the inference of demography and selection from time course data. Utilizing these novel statistical and computational tools, we evaluate whole-genome datasets of an influenza A H1N1 strain in the presence and absence of oseltamivir (an inhibitor of neuraminidase) collected at thirteen time points. Results reveal a striking consistency amongst the three estimation procedures developed, showing strongly increased selection pressure in the presence of drug treatment. Importantly, these approaches re-identify the known oseltamivir resistance site, successfully validating the approaches used. Enticingly, a number of previously unknown variants have also been identified as being positively selected. Results are interpreted in the light of Fisher's Geometric Model, allowing for a quantification of the increased distance to optimum exerted by the presence of drug, and theoretical predictions regarding the distribution of beneficial fitness effects of contending mutations are empirically tested. Further, given the fit to expectations of the Geometric Model, results suggest the ability to predict certain aspects of viral evolution in response to changing host environments and novel selective pressures.
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publisher Public Library of Science (PLoS)
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spelling doaj-art-c83d55bae4ba41bfac40d6f845e6e97e2025-08-20T03:01:14ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042014-02-01102e100418510.1371/journal.pgen.1004185Influenza virus drug resistance: a time-sampled population genetics perspective.Matthieu FollYu-Ping PohNicholas RenzetteAnna Ferrer-AdmetllaClaudia BankHyunjin ShimAnna-Sapfo MalaspinasGregory EwingPing LiuDaniel WegmannDaniel R CaffreyKonstantin B ZeldovichDaniel N BolonJennifer P WangTimothy F KowalikCelia A SchifferRobert W FinbergJeffrey D JensenThe challenge of distinguishing genetic drift from selection remains a central focus of population genetics. Time-sampled data may provide a powerful tool for distinguishing these processes, and we here propose approximate Bayesian, maximum likelihood, and analytical methods for the inference of demography and selection from time course data. Utilizing these novel statistical and computational tools, we evaluate whole-genome datasets of an influenza A H1N1 strain in the presence and absence of oseltamivir (an inhibitor of neuraminidase) collected at thirteen time points. Results reveal a striking consistency amongst the three estimation procedures developed, showing strongly increased selection pressure in the presence of drug treatment. Importantly, these approaches re-identify the known oseltamivir resistance site, successfully validating the approaches used. Enticingly, a number of previously unknown variants have also been identified as being positively selected. Results are interpreted in the light of Fisher's Geometric Model, allowing for a quantification of the increased distance to optimum exerted by the presence of drug, and theoretical predictions regarding the distribution of beneficial fitness effects of contending mutations are empirically tested. Further, given the fit to expectations of the Geometric Model, results suggest the ability to predict certain aspects of viral evolution in response to changing host environments and novel selective pressures.https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1004185&type=printable
spellingShingle Matthieu Foll
Yu-Ping Poh
Nicholas Renzette
Anna Ferrer-Admetlla
Claudia Bank
Hyunjin Shim
Anna-Sapfo Malaspinas
Gregory Ewing
Ping Liu
Daniel Wegmann
Daniel R Caffrey
Konstantin B Zeldovich
Daniel N Bolon
Jennifer P Wang
Timothy F Kowalik
Celia A Schiffer
Robert W Finberg
Jeffrey D Jensen
Influenza virus drug resistance: a time-sampled population genetics perspective.
PLoS Genetics
title Influenza virus drug resistance: a time-sampled population genetics perspective.
title_full Influenza virus drug resistance: a time-sampled population genetics perspective.
title_fullStr Influenza virus drug resistance: a time-sampled population genetics perspective.
title_full_unstemmed Influenza virus drug resistance: a time-sampled population genetics perspective.
title_short Influenza virus drug resistance: a time-sampled population genetics perspective.
title_sort influenza virus drug resistance a time sampled population genetics perspective
url https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1004185&type=printable
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