Are there Risks from Nanocomposite Restoration Grinding for Dentists?
Objectives: To evaluate the effect of short-term inhalational exposure to nanoparticles released during dental composite grinding on oxidative stress and antioxidant capacity markers. Materials and methods: Twenty-four healthy volunteers were examined before and after exposure in dental workshop. Th...
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| Language: | English |
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Elsevier
2025-02-01
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| Series: | International Dental Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0020653924001448 |
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| author | Daniela Pelclova Pavel Bradna Lucie Lischkova Vladimir Zdimal Ludmila Maskova Pavlina Klusackova Viktoriia Kolesnikova Jakub Ondracek Jaroslav Schwarz Miroslav Pohanka Tomas Navratil Stepanka Vlckova Zdenka Fenclova Jana Duskova Andrea Rossnerova Adela Roubickova |
| author_facet | Daniela Pelclova Pavel Bradna Lucie Lischkova Vladimir Zdimal Ludmila Maskova Pavlina Klusackova Viktoriia Kolesnikova Jakub Ondracek Jaroslav Schwarz Miroslav Pohanka Tomas Navratil Stepanka Vlckova Zdenka Fenclova Jana Duskova Andrea Rossnerova Adela Roubickova |
| author_sort | Daniela Pelclova |
| collection | DOAJ |
| description | Objectives: To evaluate the effect of short-term inhalational exposure to nanoparticles released during dental composite grinding on oxidative stress and antioxidant capacity markers. Materials and methods: Twenty-four healthy volunteers were examined before and after exposure in dental workshop. They spent 76.8 ± 0.7 min in the testing room during grinding of dental nanocomposites. The individual exposure to aerosol particles in each participant´s breathing zones was monitored using a personal nanoparticle sampler (PENS). Exhaled breath condensate (EBC), blood, and urine samples were collected pre- and post-exposure to measure one oxidative stress marker, i.e., thiobarbituric acid reactive substances (TBARS), and two biomarkers of antioxidant capacity, i.e., ferric-reducing antioxidant power (FRAP) and reduced glutathione (GSH) by spectrophotometry. Spirometry and fractional exhaled nitric oxide (FeNO) were used to evaluate the effect of acute inhalational exposure. Results: Mean mass of dental nanocomposite ground away was 0.88 ± 0.32 g. Average individual doses of respirable particles and nanoparticles measured by PENS were 380 ± 150 and 3.3 ± 1.3 μg, respectively. No significant increase of the post-exposure oxidative stress marker TBARS in EBC and plasma was seen. No decrease in antioxidant capacity biomarkers FRAP and GSH in EBC post-exposure was seen, either. Post-exposure, conjunctival hyperemia was seen in 62.5% volunteers; however, no impairment in spirometry or FeNO results was observed. No correlation of any biomarker measured with individual exposure was found, however, several correlations with interfering factors (age, body mass index, hypertension, dyslipidemia, and environmental pollution parameters) were seen. Conclusions: This study, using oxidative stress biomarker and antioxidant capacity biomarkers in biological fluids of volunteers during the grinding of dental nanocomposites did not prove a negative effect of this intense short-term exposure. However, further studies are needed to evaluate oxidative stress in long-term exposure of both stomatologists and patients and diverse populations with varying health statuses. |
| format | Article |
| id | doaj-art-c83215e6801e4cc49c044800fffb17ff |
| institution | Kabale University |
| issn | 0020-6539 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Dental Journal |
| spelling | doaj-art-c83215e6801e4cc49c044800fffb17ff2025-01-21T04:12:41ZengElsevierInternational Dental Journal0020-65392025-02-01751305313Are there Risks from Nanocomposite Restoration Grinding for Dentists?Daniela Pelclova0Pavel Bradna1Lucie Lischkova2Vladimir Zdimal3Ludmila Maskova4Pavlina Klusackova5Viktoriia Kolesnikova6Jakub Ondracek7Jaroslav Schwarz8Miroslav Pohanka9Tomas Navratil10Stepanka Vlckova11Zdenka Fenclova12Jana Duskova13Andrea Rossnerova14Adela Roubickova15Department of Occupational Medicine, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Prague, Czech Republic; Corresponding author. Department of Occupational Medicine, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Na Bojisti 1, 12000 Prague, Czech Republic.Institute of Dental Medicine, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Prague, Czech RepublicDepartment of Occupational Medicine, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Prague, Czech RepublicDepartment of Aerosol Chemistry and Physics, Institute of Chemical Process Fundamentals of the Czech Academy of Sciences, Prague, Czech RepublicDepartment of Aerosol Chemistry and Physics, Institute of Chemical Process Fundamentals of the Czech Academy of Sciences, Prague, Czech RepublicDepartment of Occupational Medicine, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Prague, Czech RepublicDepartment of Occupational Medicine, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Prague, Czech RepublicDepartment of Aerosol Chemistry and Physics, Institute of Chemical Process Fundamentals of the Czech Academy of Sciences, Prague, Czech RepublicDepartment of Aerosol Chemistry and Physics, Institute of Chemical Process Fundamentals of the Czech Academy of Sciences, Prague, Czech RepublicFaculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech RepublicJ. Heyrovsky Institute of Physical Chemistry of the Czech Academy of Sciences, Prague, Czech RepublicDepartment of Occupational Medicine, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Prague, Czech RepublicDepartment of Occupational Medicine, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Prague, Czech RepublicInstitute of Dental Medicine, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Prague, Czech RepublicInstitute of Experimental Medicine of the Czech Academy of Sciences, Department of Nanotoxicology and Molecular Epidemiology, Prague, Czech RepublicInstitute of Dental Medicine, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Prague, Czech RepublicObjectives: To evaluate the effect of short-term inhalational exposure to nanoparticles released during dental composite grinding on oxidative stress and antioxidant capacity markers. Materials and methods: Twenty-four healthy volunteers were examined before and after exposure in dental workshop. They spent 76.8 ± 0.7 min in the testing room during grinding of dental nanocomposites. The individual exposure to aerosol particles in each participant´s breathing zones was monitored using a personal nanoparticle sampler (PENS). Exhaled breath condensate (EBC), blood, and urine samples were collected pre- and post-exposure to measure one oxidative stress marker, i.e., thiobarbituric acid reactive substances (TBARS), and two biomarkers of antioxidant capacity, i.e., ferric-reducing antioxidant power (FRAP) and reduced glutathione (GSH) by spectrophotometry. Spirometry and fractional exhaled nitric oxide (FeNO) were used to evaluate the effect of acute inhalational exposure. Results: Mean mass of dental nanocomposite ground away was 0.88 ± 0.32 g. Average individual doses of respirable particles and nanoparticles measured by PENS were 380 ± 150 and 3.3 ± 1.3 μg, respectively. No significant increase of the post-exposure oxidative stress marker TBARS in EBC and plasma was seen. No decrease in antioxidant capacity biomarkers FRAP and GSH in EBC post-exposure was seen, either. Post-exposure, conjunctival hyperemia was seen in 62.5% volunteers; however, no impairment in spirometry or FeNO results was observed. No correlation of any biomarker measured with individual exposure was found, however, several correlations with interfering factors (age, body mass index, hypertension, dyslipidemia, and environmental pollution parameters) were seen. Conclusions: This study, using oxidative stress biomarker and antioxidant capacity biomarkers in biological fluids of volunteers during the grinding of dental nanocomposites did not prove a negative effect of this intense short-term exposure. However, further studies are needed to evaluate oxidative stress in long-term exposure of both stomatologists and patients and diverse populations with varying health statuses.http://www.sciencedirect.com/science/article/pii/S0020653924001448NanoparticlesDental nanocompositeExhaled airway markersRespiratory function testOccupational lung disease |
| spellingShingle | Daniela Pelclova Pavel Bradna Lucie Lischkova Vladimir Zdimal Ludmila Maskova Pavlina Klusackova Viktoriia Kolesnikova Jakub Ondracek Jaroslav Schwarz Miroslav Pohanka Tomas Navratil Stepanka Vlckova Zdenka Fenclova Jana Duskova Andrea Rossnerova Adela Roubickova Are there Risks from Nanocomposite Restoration Grinding for Dentists? International Dental Journal Nanoparticles Dental nanocomposite Exhaled airway markers Respiratory function test Occupational lung disease |
| title | Are there Risks from Nanocomposite Restoration Grinding for Dentists? |
| title_full | Are there Risks from Nanocomposite Restoration Grinding for Dentists? |
| title_fullStr | Are there Risks from Nanocomposite Restoration Grinding for Dentists? |
| title_full_unstemmed | Are there Risks from Nanocomposite Restoration Grinding for Dentists? |
| title_short | Are there Risks from Nanocomposite Restoration Grinding for Dentists? |
| title_sort | are there risks from nanocomposite restoration grinding for dentists |
| topic | Nanoparticles Dental nanocomposite Exhaled airway markers Respiratory function test Occupational lung disease |
| url | http://www.sciencedirect.com/science/article/pii/S0020653924001448 |
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