Mapping the microbial diversity associated with mango fruit during postharvest handling, cold plasma treatment, storage and after drying
Postharvest treatments have a central impact on the microbial community of mango fruit. This study investigated the dynamics of the bacterial community on ‘Heidi’ mango fruit surfaces from harvest (on tree in the farm) to cold storage, pre-treatments and processing (drying at 60 °C) using 16S rRNA-b...
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Elsevier
2025-12-01
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| Series: | Applied Food Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772502225004093 |
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| author | Loriane A. Yanclo Alvera Vorster Carel Van Heerden Gunnar Sigge Zinash A. Belay Oluwafemi James Caleb |
| author_facet | Loriane A. Yanclo Alvera Vorster Carel Van Heerden Gunnar Sigge Zinash A. Belay Oluwafemi James Caleb |
| author_sort | Loriane A. Yanclo |
| collection | DOAJ |
| description | Postharvest treatments have a central impact on the microbial community of mango fruit. This study investigated the dynamics of the bacterial community on ‘Heidi’ mango fruit surfaces from harvest (on tree in the farm) to cold storage, pre-treatments and processing (drying at 60 °C) using 16S rRNA-based metagenomic sequencing. A total of 544,965 reads belonging to 20 bacteria phyla were associated with freshly harvested mango surface. At harvest, Proteobacteria were the dominant phylum (61%), followed by Actinobacteria (20%), Cyanobacteria (9%), and Firmicutes (3%). Transportation resulted a 92% abundance of Proteobacteria. Pre-treatment of whole fruit with low pressure cold plasma (CP) for 5 min (CP5) and 10 min (CP10), and fungicide (Chronos Prochloraz) significantly altered the bacterial community. This study showed that the abundance of Proteobacteria on whole fruit was highest for CP10 (77%) followed by Chronos Prochloraz (45%) after 4 weeks of cool storage. Cyanobacteria were eliminated in dried fresh-cut mango slices with the combination of plasma jet pre-treatment and drying. This work provides evidence to support the potential application of next generation sequencing in distinguishing various bacterial communities along the entire postharvest value chain. |
| format | Article |
| id | doaj-art-c8301278398f4c3cbde3e3878a04ecc0 |
| institution | OA Journals |
| issn | 2772-5022 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Applied Food Research |
| spelling | doaj-art-c8301278398f4c3cbde3e3878a04ecc02025-08-20T02:37:45ZengElsevierApplied Food Research2772-50222025-12-015210110110.1016/j.afres.2025.101101Mapping the microbial diversity associated with mango fruit during postharvest handling, cold plasma treatment, storage and after dryingLoriane A. Yanclo0Alvera Vorster1Carel Van Heerden2Gunnar Sigge3Zinash A. Belay4Oluwafemi James Caleb5Department of Food Science, Faculty of AgriSciences, Stellenbosch University, Private Bag X1, Stellenbosch 7600, South Africa; AgriFood BioSystems and Technovation Research Group, Africa Institute for Postharvest Technology, Faculty of AgriSciences, Stellenbosch University, Private Bag X1, Stellenbosch 7600, South AfricaCentral Analytical Facilities (CAF), the DNA-Sequencing Unit, Stellenbosch University, Private Bag X1, Stellenbosch 7602, South AfricaCentral Analytical Facilities (CAF), the DNA-Sequencing Unit, Stellenbosch University, Private Bag X1, Stellenbosch 7602, South AfricaDepartment of Food Science, Faculty of AgriSciences, Stellenbosch University, Private Bag X1, Stellenbosch 7600, South AfricaDepartment of Food Science, Faculty of AgriSciences, Stellenbosch University, Private Bag X1, Stellenbosch 7600, South Africa; Agri-Food Systems and Omics Laboratory, Post-Harvest and Agro-Processing Technologies (PHATs), Agricultural Research Council (ARC) Infruitec-Nietvoorbij, Private Bag X5026, Stellenbosch 7599, South AfricaDepartment of Food Science, Faculty of AgriSciences, Stellenbosch University, Private Bag X1, Stellenbosch 7600, South Africa; AgriFood BioSystems and Technovation Research Group, Africa Institute for Postharvest Technology, Faculty of AgriSciences, Stellenbosch University, Private Bag X1, Stellenbosch 7600, South Africa; Corresponding author at: Department of Food Science, Faculty of AgriSciences, Stellenbosch University, Private Bag X1, Stellenbosch 7600, South Africa.Postharvest treatments have a central impact on the microbial community of mango fruit. This study investigated the dynamics of the bacterial community on ‘Heidi’ mango fruit surfaces from harvest (on tree in the farm) to cold storage, pre-treatments and processing (drying at 60 °C) using 16S rRNA-based metagenomic sequencing. A total of 544,965 reads belonging to 20 bacteria phyla were associated with freshly harvested mango surface. At harvest, Proteobacteria were the dominant phylum (61%), followed by Actinobacteria (20%), Cyanobacteria (9%), and Firmicutes (3%). Transportation resulted a 92% abundance of Proteobacteria. Pre-treatment of whole fruit with low pressure cold plasma (CP) for 5 min (CP5) and 10 min (CP10), and fungicide (Chronos Prochloraz) significantly altered the bacterial community. This study showed that the abundance of Proteobacteria on whole fruit was highest for CP10 (77%) followed by Chronos Prochloraz (45%) after 4 weeks of cool storage. Cyanobacteria were eliminated in dried fresh-cut mango slices with the combination of plasma jet pre-treatment and drying. This work provides evidence to support the potential application of next generation sequencing in distinguishing various bacterial communities along the entire postharvest value chain.http://www.sciencedirect.com/science/article/pii/S2772502225004093DNA sequencingDried fruit mango slicesPhylumNon-thermal treatment |
| spellingShingle | Loriane A. Yanclo Alvera Vorster Carel Van Heerden Gunnar Sigge Zinash A. Belay Oluwafemi James Caleb Mapping the microbial diversity associated with mango fruit during postharvest handling, cold plasma treatment, storage and after drying Applied Food Research DNA sequencing Dried fruit mango slices Phylum Non-thermal treatment |
| title | Mapping the microbial diversity associated with mango fruit during postharvest handling, cold plasma treatment, storage and after drying |
| title_full | Mapping the microbial diversity associated with mango fruit during postharvest handling, cold plasma treatment, storage and after drying |
| title_fullStr | Mapping the microbial diversity associated with mango fruit during postharvest handling, cold plasma treatment, storage and after drying |
| title_full_unstemmed | Mapping the microbial diversity associated with mango fruit during postharvest handling, cold plasma treatment, storage and after drying |
| title_short | Mapping the microbial diversity associated with mango fruit during postharvest handling, cold plasma treatment, storage and after drying |
| title_sort | mapping the microbial diversity associated with mango fruit during postharvest handling cold plasma treatment storage and after drying |
| topic | DNA sequencing Dried fruit mango slices Phylum Non-thermal treatment |
| url | http://www.sciencedirect.com/science/article/pii/S2772502225004093 |
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