The baseline circulating immunophenotype characteristics associate with PD(L)-1 targeted treatment response, irae onset, and prognosis

Abstract More promising, effective, and less-invasive biomarkers for PD(L)-1 targeted responses, immune-related adverse events (irAEs), and prognosis are being explored. We conducted a single-center retrospective study in pan-cancer patients with anti-PD(L)-1 monotherapy. Observational endpoints inc...

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Main Authors: Xiaomin Fu, Fanghui Li, Hongqin You, Benling Xu, Tingjie Wang, Peng Qin, Lu Han, Yong Zhang, Fang Zhang, Lingdi Zhao, Baozhen Ma, Yiman Shang, Yonghao Yang, Zibing Wang, Jinrong Qu, Quanli Gao
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Language:English
Published: Nature Portfolio 2025-05-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-02597-5
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author Xiaomin Fu
Fanghui Li
Hongqin You
Benling Xu
Tingjie Wang
Peng Qin
Lu Han
Yong Zhang
Fang Zhang
Lingdi Zhao
Baozhen Ma
Yiman Shang
Yonghao Yang
Zibing Wang
Jinrong Qu
Quanli Gao
author_facet Xiaomin Fu
Fanghui Li
Hongqin You
Benling Xu
Tingjie Wang
Peng Qin
Lu Han
Yong Zhang
Fang Zhang
Lingdi Zhao
Baozhen Ma
Yiman Shang
Yonghao Yang
Zibing Wang
Jinrong Qu
Quanli Gao
author_sort Xiaomin Fu
collection DOAJ
description Abstract More promising, effective, and less-invasive biomarkers for PD(L)-1 targeted responses, immune-related adverse events (irAEs), and prognosis are being explored. We conducted a single-center retrospective study in pan-cancer patients with anti-PD(L)-1 monotherapy. Observational endpoints included treatment response, prognosis, and irAEs. Peripheral blood immunophenotypes were analyzed by Flow Cytometry. 104 patients were enrolled. Higher pretreatment percentages of CD3+CD4+ Th cells were associated with both responses (HR: 6.170, P = 0.034) and prognosis (HR: 1.930, P = 0.022). The higher baseline percentage of CD16+CD56+ NK cells was positively correlated with response (HR: 3.730, P = 0.050) and negatively related to irAEs (HR: 0.460, P = 0.012). Decreased pretreatment CD3+ T cell counts were related to more irAEs (HR: 0.970, P = 0.026), while the percentage of CD3+ T cells was negatively associated with prognosis (HR: 1.930, P = 0.022). The higher baseline cell counts of CD3+CD8+ CTL, CD19+ B, and the percentage of CD19+ B cells might be related to more irAEs (P < 0.05). Significant correlation between duration of treatment (DOT) and prognosis, irAE and outcome was also confirmed (P < 0.0001). Our findings confirmed multiple baseline circulating immunophenotype characteristics were related to PD(L)-1 targeted response, irAE onset, and prognosis.
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spelling doaj-art-c7dff9181b54442cac0e490f35b693fa2025-08-20T03:08:40ZengNature PortfolioScientific Reports2045-23222025-05-0115111410.1038/s41598-025-02597-5The baseline circulating immunophenotype characteristics associate with PD(L)-1 targeted treatment response, irae onset, and prognosisXiaomin Fu0Fanghui Li1Hongqin You2Benling Xu3Tingjie Wang4Peng Qin5Lu Han6Yong Zhang7Fang Zhang8Lingdi Zhao9Baozhen Ma10Yiman Shang11Yonghao Yang12Zibing Wang13Jinrong Qu14Quanli Gao15Department of Immunotherapy, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer HospitalDepartment of Immunotherapy, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer HospitalGMP Laboratory of Immunotherapy, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer HospitalGMP Laboratory of Immunotherapy, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer HospitalDepartment of Molecular Pathology, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer HospitalGMP Laboratory of Immunotherapy, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer HospitalGMP Laboratory of Immunotherapy, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer HospitalDepartment of Immunotherapy, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer HospitalDepartment of Immunotherapy, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer HospitalDepartment of Immunotherapy, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer HospitalDepartment of Immunotherapy, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer HospitalDepartment of Immunotherapy, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer HospitalDepartment of Immunotherapy, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer HospitalDepartment of Immunotherapy, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer HospitalDepartment of Radiology, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer HospitalDepartment of Immunotherapy, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer HospitalAbstract More promising, effective, and less-invasive biomarkers for PD(L)-1 targeted responses, immune-related adverse events (irAEs), and prognosis are being explored. We conducted a single-center retrospective study in pan-cancer patients with anti-PD(L)-1 monotherapy. Observational endpoints included treatment response, prognosis, and irAEs. Peripheral blood immunophenotypes were analyzed by Flow Cytometry. 104 patients were enrolled. Higher pretreatment percentages of CD3+CD4+ Th cells were associated with both responses (HR: 6.170, P = 0.034) and prognosis (HR: 1.930, P = 0.022). The higher baseline percentage of CD16+CD56+ NK cells was positively correlated with response (HR: 3.730, P = 0.050) and negatively related to irAEs (HR: 0.460, P = 0.012). Decreased pretreatment CD3+ T cell counts were related to more irAEs (HR: 0.970, P = 0.026), while the percentage of CD3+ T cells was negatively associated with prognosis (HR: 1.930, P = 0.022). The higher baseline cell counts of CD3+CD8+ CTL, CD19+ B, and the percentage of CD19+ B cells might be related to more irAEs (P < 0.05). Significant correlation between duration of treatment (DOT) and prognosis, irAE and outcome was also confirmed (P < 0.0001). Our findings confirmed multiple baseline circulating immunophenotype characteristics were related to PD(L)-1 targeted response, irAE onset, and prognosis.https://doi.org/10.1038/s41598-025-02597-5PD(L)-1 inhibitorMonotherapyResponseIrAEPrognosisBiomarker
spellingShingle Xiaomin Fu
Fanghui Li
Hongqin You
Benling Xu
Tingjie Wang
Peng Qin
Lu Han
Yong Zhang
Fang Zhang
Lingdi Zhao
Baozhen Ma
Yiman Shang
Yonghao Yang
Zibing Wang
Jinrong Qu
Quanli Gao
The baseline circulating immunophenotype characteristics associate with PD(L)-1 targeted treatment response, irae onset, and prognosis
Scientific Reports
PD(L)-1 inhibitor
Monotherapy
Response
IrAE
Prognosis
Biomarker
title The baseline circulating immunophenotype characteristics associate with PD(L)-1 targeted treatment response, irae onset, and prognosis
title_full The baseline circulating immunophenotype characteristics associate with PD(L)-1 targeted treatment response, irae onset, and prognosis
title_fullStr The baseline circulating immunophenotype characteristics associate with PD(L)-1 targeted treatment response, irae onset, and prognosis
title_full_unstemmed The baseline circulating immunophenotype characteristics associate with PD(L)-1 targeted treatment response, irae onset, and prognosis
title_short The baseline circulating immunophenotype characteristics associate with PD(L)-1 targeted treatment response, irae onset, and prognosis
title_sort baseline circulating immunophenotype characteristics associate with pd l 1 targeted treatment response irae onset and prognosis
topic PD(L)-1 inhibitor
Monotherapy
Response
IrAE
Prognosis
Biomarker
url https://doi.org/10.1038/s41598-025-02597-5
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