Accurate phenotype-to-genotype mapping of high-diversity yeast libraries by heat-shock-electroporation (HEEL)

Accurate phenotype-to-genotype mapping of high-diversity yeast libraries by heat-shock-electroporation (HEEL)

ABSTRACT High-throughput DNA transformation techniques are invaluable when generating high-diversity mutant libraries, a cornerstone of successful protein engineering. However, transformation efficiencies have a direct correlation with the probability of introducing multiple DNA molecules into each...

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Bibliographic Details
Main Authors: Marcus Wäneskog, Emma Elise Hoch-Schneider, Shilpa Garg, Christian Kronborg Cantalapiedra, Elena Schäfer, Michael Krogh Jensen, Emil Damgaard Jensen
Format: Article
Language:English
Published: American Society for Microbiology 2025-02-01
Series:mBio
Subjects:
DNA transformation
DNA electroporation
automated genotyping
heat-shock
barcodes
yeast
Online Access:https://journals.asm.org/doi/10.1128/mbio.03197-24
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https://journals.asm.org/doi/10.1128/mbio.03197-24

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