Direct cardiac reprogramming via combined CRISPRa-mediated endogenous Gata4 activation and exogenous Mef2c and Tbx5 expression

Direct cardiac reprogramming via combined CRISPRa-mediated endogenous Gata4 activation and exogenous Mef2c and Tbx5 expression

Direct cardiac reprogramming of fibroblasts into induced cardiomyocytes (iCMs) can be achieved by ectopic expression of cardiac transcription factors (TFs) via viral vectors. However, risks like genomic mutations, viral toxicity, and immune response limited its clinical application. Transactivation...

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Bibliographic Details
Main Authors: Peisen Huang, Jun Xu, Benjamin Keepers, Yifang Xie, David Near, Yangxi Xu, James Rock Hua, Brian Spurlock, Shea Ricketts, Jiandong Liu, Li Wang, Li Qian
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Molecular Therapy: Nucleic Acids
Subjects:
MT: RNA/DNA Editing
guide RNA
CRISPRdCas9 system
endogenous transactivation
direct cardiac reprogramming
MGT
Online Access:http://www.sciencedirect.com/science/article/pii/S2162253124002774
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