insilico.mutagenesis: a primer selection tool designed for sequence scanning applications used in directed evolution experiments
Several primer prediction programs have been developed for a variety of applications. However, none of these tools allows the prediction of a large set of primers for whole gene site-directed mutagenesis experiments using the megaprimer method. We report a novel primer prediction tool (insilico.muta...
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| Main Authors: | Ulrich Krauss, Thorsten Eggert |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2005-11-01
|
| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/000112013 |
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