insilico.mutagenesis: a primer selection tool designed for sequence scanning applications used in directed evolution experiments

Several primer prediction programs have been developed for a variety of applications. However, none of these tools allows the prediction of a large set of primers for whole gene site-directed mutagenesis experiments using the megaprimer method. We report a novel primer prediction tool (insilico.muta...

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Bibliographic Details
Main Authors: Ulrich Krauss, Thorsten Eggert
Format: Article
Language:English
Published: Taylor & Francis Group 2005-11-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/000112013
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