Room temperature CRISPR diagnostics for low-resource settings

Room temperature CRISPR diagnostics for low-resource settings

Abstract Maintaining elevated reaction temperatures and multi-step sample preparations increases the costs and complexity of diagnostics, impeding their deployment in low-resource settings. Here, we develop a one-pot, room temperature recombinase polymerase amplification (RPA)-CRISPR reaction that r...

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Main Authors: Eric A. Nalefski, Selma Sinan, Jason L. Cantera, Anastasia G. Kim, Remy M. Kooistra, Rafael E. Rivera, Jordyn P. Janshen, Sanchita Bhadra, Joshua D. Bishop, Andrew D. Ellington, Ilya J. Finklestein, Damian Madan
Format: Article
Language:English
Published: Nature Portfolio 2025-01-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-025-86373-5
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https://doi.org/10.1038/s41598-025-86373-5

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