Development of a human tyrosinase activity inhibition assay using human melanoma cell lysate
Tyrosinase (TYR) inhibitors are required to treat skin hyperpigmentation. Currently live cell-based TYR assays and mushroom TYR in vitro assays are the common methods used to screen for TYR inhibitors. However, these methods are either time consuming and expensive or are not human TYR (hsTYR) specif...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-11-01
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| Series: | BioTechniques |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/07366205.2024.2441637 |
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| author | Gengxuan Shi Yunjiang Feng Kathryn F. Tonissen |
| author_facet | Gengxuan Shi Yunjiang Feng Kathryn F. Tonissen |
| author_sort | Gengxuan Shi |
| collection | DOAJ |
| description | Tyrosinase (TYR) inhibitors are required to treat skin hyperpigmentation. Currently live cell-based TYR assays and mushroom TYR in vitro assays are the common methods used to screen for TYR inhibitors. However, these methods are either time consuming and expensive or are not human TYR (hsTYR) specific. Here, we describe a simple hsTYR assay using cell lysate prepared from pigmented human melanoma cell lines that takes less than 3 hours to complete after collecting cell pellets. We confirmed the assay is species specific by using a known hsTYR inhibitor, kojic acid, as a positive control, while arbutin, which inhibits mushroom TYR, but not hsTYR, was not effective. This assay is a simple method to confirm hsTYR inhibition before conducting follow-up studies in live biological models. |
| format | Article |
| id | doaj-art-c5f16eddbdb949178400277ca071d1dc |
| institution | Kabale University |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-c5f16eddbdb949178400277ca071d1dc2025-01-21T13:10:56ZengTaylor & Francis GroupBioTechniques0736-62051940-98182024-11-01761154755110.1080/07366205.2024.2441637Development of a human tyrosinase activity inhibition assay using human melanoma cell lysateGengxuan Shi0Yunjiang Feng1Kathryn F. Tonissen2Institute for Biomedicine and Glycomics and School of Environment and Science, Griffith University, Nathan, Qld, 4111, AustraliaInstitute for Biomedicine and Glycomics and School of Environment and Science, Griffith University, Nathan, Qld, 4111, AustraliaInstitute for Biomedicine and Glycomics and School of Environment and Science, Griffith University, Nathan, Qld, 4111, AustraliaTyrosinase (TYR) inhibitors are required to treat skin hyperpigmentation. Currently live cell-based TYR assays and mushroom TYR in vitro assays are the common methods used to screen for TYR inhibitors. However, these methods are either time consuming and expensive or are not human TYR (hsTYR) specific. Here, we describe a simple hsTYR assay using cell lysate prepared from pigmented human melanoma cell lines that takes less than 3 hours to complete after collecting cell pellets. We confirmed the assay is species specific by using a known hsTYR inhibitor, kojic acid, as a positive control, while arbutin, which inhibits mushroom TYR, but not hsTYR, was not effective. This assay is a simple method to confirm hsTYR inhibition before conducting follow-up studies in live biological models.https://www.tandfonline.com/doi/10.1080/07366205.2024.2441637Human tyrosinase inhibitorskojic acidarbutinskin pigmentationmelanoma cell lysatetyrosinase |
| spellingShingle | Gengxuan Shi Yunjiang Feng Kathryn F. Tonissen Development of a human tyrosinase activity inhibition assay using human melanoma cell lysate BioTechniques Human tyrosinase inhibitors kojic acid arbutin skin pigmentation melanoma cell lysate tyrosinase |
| title | Development of a human tyrosinase activity inhibition assay using human melanoma cell lysate |
| title_full | Development of a human tyrosinase activity inhibition assay using human melanoma cell lysate |
| title_fullStr | Development of a human tyrosinase activity inhibition assay using human melanoma cell lysate |
| title_full_unstemmed | Development of a human tyrosinase activity inhibition assay using human melanoma cell lysate |
| title_short | Development of a human tyrosinase activity inhibition assay using human melanoma cell lysate |
| title_sort | development of a human tyrosinase activity inhibition assay using human melanoma cell lysate |
| topic | Human tyrosinase inhibitors kojic acid arbutin skin pigmentation melanoma cell lysate tyrosinase |
| url | https://www.tandfonline.com/doi/10.1080/07366205.2024.2441637 |
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