Phage Display Shot-Gun Cloning of Ligand-Binding Domains of Prokaryotic Receptors Approaches 100% Correct Clones
We recently presented an application of the phage display technique enabling cloning of DNA encoding ligand-binding domain(s) of prokaryotic receptors directly from chromosomal DNA. Here we show that the use of a gene VIII-based, instead of a gene III-based, phagemid vector system results in a much...
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| Main Authors: | Karin Jacobsson, Lars Frykberg |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1996-06-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/96206rr04 |
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