Phage Display Shot-Gun Cloning of Ligand-Binding Domains of Prokaryotic Receptors Approaches 100% Correct Clones

We recently presented an application of the phage display technique enabling cloning of DNA encoding ligand-binding domain(s) of prokaryotic receptors directly from chromosomal DNA. Here we show that the use of a gene VIII-based, instead of a gene III-based, phagemid vector system results in a much...

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Bibliographic Details
Main Authors: Karin Jacobsson, Lars Frykberg
Format: Article
Language:English
Published: Taylor & Francis Group 1996-06-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/96206rr04
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