Farnesoid X Receptor Regulated Sepsis‐Induced Abnormal Bile Acid Metabolism via the Fibroblast Growth Factor 15/Fibroblast Growth Factor Receptor 4 Pathway
Abstract Objective The study aims to investigate the mechanism of Farnesoid X receptor (FXR) activation in sepsis‐induced abnormal bile acid metabolism and the metabolism status of each bile acid type. Methods The sepsis mouse model was developed via lipopolysaccharide intraperitoneal injection and...
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| Format: | Article |
| Language: | English |
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Wiley
2025-04-01
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| Series: | Immunity, Inflammation and Disease |
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| Online Access: | https://doi.org/10.1002/iid3.70155 |
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| author | Ziyang Zhou Dan Xu Liou Huang Yuhui Cui Hui Chen Jianguo Tang |
| author_facet | Ziyang Zhou Dan Xu Liou Huang Yuhui Cui Hui Chen Jianguo Tang |
| author_sort | Ziyang Zhou |
| collection | DOAJ |
| description | Abstract Objective The study aims to investigate the mechanism of Farnesoid X receptor (FXR) activation in sepsis‐induced abnormal bile acid metabolism and the metabolism status of each bile acid type. Methods The sepsis mouse model was developed via lipopolysaccharide intraperitoneal injection and confirmed via hematoxylin and eosin (H&E) staining. FXR agonist activated the FXR/fibroblast growth factor (FGF)15/FGFR pathway via quantitative real‐time polymerase chain reaction and Western blot. Consequently, metabolomics and bioinformatics analysis were conducted to identify the alterations in each kind of bile acid content following FXR agonist/inhibitor intervention. Results The H&E staining indicated that FXR activation alleviates the liver injury of the sepsis mouse model. The increased FGF15 and FXFR expression levels and decreased CYP7A1 demonstrated FXR/FGF15/FGFR pathway activation following FXR agonist treatment. Furthermore, total bile acid, interleukin (IL)‐6, and tumor necrosis factor‐α concentrations were downregulated after FXR activation, whereas IL‐10 concentration was upregulated, indicating the alleviated effect of FXR agonist in sepsis. Consequently, metabolomics and bioinformatics analysis determined that T‐a‐MCA were downregulated in both FXR agonist and inhibitor groups, whereas six bile acid types were altered in the control group. Conclusion FXR activation was crucial in alleviating sepsis‐induced hepatic injury and cholestasis through the FGF15/FGFR signaling pathway, and FXR may act as a potential preventive and intervention target of sepsis. |
| format | Article |
| id | doaj-art-c48ee9b300164533afef0dcf6a5e6ede |
| institution | OA Journals |
| issn | 2050-4527 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Wiley |
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| series | Immunity, Inflammation and Disease |
| spelling | doaj-art-c48ee9b300164533afef0dcf6a5e6ede2025-08-20T02:28:44ZengWileyImmunity, Inflammation and Disease2050-45272025-04-01134n/an/a10.1002/iid3.70155Farnesoid X Receptor Regulated Sepsis‐Induced Abnormal Bile Acid Metabolism via the Fibroblast Growth Factor 15/Fibroblast Growth Factor Receptor 4 PathwayZiyang Zhou0Dan Xu1Liou Huang2Yuhui Cui3Hui Chen4Jianguo Tang5Trauma‐Emergency & Critical Care Medicine Center Shanghai Fifth People's Hospital Affiliated to Fudan University Shanghai ChinaTrauma‐Emergency & Critical Care Medicine Center Shanghai Fifth People's Hospital Affiliated to Fudan University Shanghai ChinaTrauma‐Emergency & Critical Care Medicine Center Shanghai Fifth People's Hospital Affiliated to Fudan University Shanghai ChinaTrauma‐Emergency & Critical Care Medicine Center Shanghai Fifth People's Hospital Affiliated to Fudan University Shanghai ChinaJoint Center for Translational Medicine, Shanghai Fifth People's Hospital, Fudan University and School of Life Science East China Normal University Shanghai ChinaTrauma‐Emergency & Critical Care Medicine Center Shanghai Fifth People's Hospital Affiliated to Fudan University Shanghai ChinaAbstract Objective The study aims to investigate the mechanism of Farnesoid X receptor (FXR) activation in sepsis‐induced abnormal bile acid metabolism and the metabolism status of each bile acid type. Methods The sepsis mouse model was developed via lipopolysaccharide intraperitoneal injection and confirmed via hematoxylin and eosin (H&E) staining. FXR agonist activated the FXR/fibroblast growth factor (FGF)15/FGFR pathway via quantitative real‐time polymerase chain reaction and Western blot. Consequently, metabolomics and bioinformatics analysis were conducted to identify the alterations in each kind of bile acid content following FXR agonist/inhibitor intervention. Results The H&E staining indicated that FXR activation alleviates the liver injury of the sepsis mouse model. The increased FGF15 and FXFR expression levels and decreased CYP7A1 demonstrated FXR/FGF15/FGFR pathway activation following FXR agonist treatment. Furthermore, total bile acid, interleukin (IL)‐6, and tumor necrosis factor‐α concentrations were downregulated after FXR activation, whereas IL‐10 concentration was upregulated, indicating the alleviated effect of FXR agonist in sepsis. Consequently, metabolomics and bioinformatics analysis determined that T‐a‐MCA were downregulated in both FXR agonist and inhibitor groups, whereas six bile acid types were altered in the control group. Conclusion FXR activation was crucial in alleviating sepsis‐induced hepatic injury and cholestasis through the FGF15/FGFR signaling pathway, and FXR may act as a potential preventive and intervention target of sepsis.https://doi.org/10.1002/iid3.70155bile acidfarnesoid X receptorFGF15/FXFR pathwaymetabolomicssepsis |
| spellingShingle | Ziyang Zhou Dan Xu Liou Huang Yuhui Cui Hui Chen Jianguo Tang Farnesoid X Receptor Regulated Sepsis‐Induced Abnormal Bile Acid Metabolism via the Fibroblast Growth Factor 15/Fibroblast Growth Factor Receptor 4 Pathway Immunity, Inflammation and Disease bile acid farnesoid X receptor FGF15/FXFR pathway metabolomics sepsis |
| title | Farnesoid X Receptor Regulated Sepsis‐Induced Abnormal Bile Acid Metabolism via the Fibroblast Growth Factor 15/Fibroblast Growth Factor Receptor 4 Pathway |
| title_full | Farnesoid X Receptor Regulated Sepsis‐Induced Abnormal Bile Acid Metabolism via the Fibroblast Growth Factor 15/Fibroblast Growth Factor Receptor 4 Pathway |
| title_fullStr | Farnesoid X Receptor Regulated Sepsis‐Induced Abnormal Bile Acid Metabolism via the Fibroblast Growth Factor 15/Fibroblast Growth Factor Receptor 4 Pathway |
| title_full_unstemmed | Farnesoid X Receptor Regulated Sepsis‐Induced Abnormal Bile Acid Metabolism via the Fibroblast Growth Factor 15/Fibroblast Growth Factor Receptor 4 Pathway |
| title_short | Farnesoid X Receptor Regulated Sepsis‐Induced Abnormal Bile Acid Metabolism via the Fibroblast Growth Factor 15/Fibroblast Growth Factor Receptor 4 Pathway |
| title_sort | farnesoid x receptor regulated sepsis induced abnormal bile acid metabolism via the fibroblast growth factor 15 fibroblast growth factor receptor 4 pathway |
| topic | bile acid farnesoid X receptor FGF15/FXFR pathway metabolomics sepsis |
| url | https://doi.org/10.1002/iid3.70155 |
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